重组大肠杆菌高效表达hbFGF的培养条件  

Study on the Cultural Conditions of High Expression of Human Basic Fibroblast Growth Factor by Recombinant Escherichia coli hbFGF/BL21(DE3)pLysS

作　　者：廖美德[1] 谢秋玲[2] 林剑[2] 洪岸[2] 孙奋勇[2] 梁世中[1] 

机构地区：[1]华南理工大学食品与生物工程学院,广东广州510640 [2]暨南大学生物工程研究所,广东广州510632

出　　处：《华南理工大学学报（自然科学版）》2002年第6期73-75,80,共4页Journal of South China University of Technology(Natural Science Edition)

基　　金：九五国家科技攻关资助项目 (96-c0 2-0 1-0 3)

摘　　要：对工程菌hbFGF/BL2 1(DE3) pLysS高效表达hbFGF的培养条件的研究结果表明 ,接种量 (φ)为 5 % ,当培养液菌体浓度为 0 .8OD6 0 0 时 ,添加 0 .5mmol/L诱导剂IPTG可以获得较高的hbFGF表达量 ,30 %的溶解氧和较低的醋酸浓度有利于hbFGF的表达 .The results of the study on the cultural conditions of high expression of hbFGF by recombinant Escherichia coli hbFGF/BL21(DE3)pLysS show that with inoculum quantity as 5% of the cultural medium, adding 0.5 mmol/L IPTG as revulsant to the leavening when its OD 600 reaches 0.8, higher expre_ ssion of hbFGF can be gained. 30% dissolved oxygen and lower concentration of acetate acid are conductive to hbFGF expression.

关 键 词：人碱性成纤维细胞生长因子(hbFGF) hbFGF/BL21(DE3)pLysS 培养条件 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]