卵巢切除大鼠股骨颈质量的力学特征与附着肌群肌强度的基因表达  

Mechanical characteristics of the femoral neck bone quality and the gene expression related to the attached muscle strength in postmenopausal osteoporotic rat model induced by ovariectomy

作　　者：张戈[1] 石印玉[1] 沈培芝[1] 徐宇[1] 郑昱新[1] 朱太泳 童普德[3] 吴小江[3] 

机构地区：[1]上海中医药大学附属曙光医院骨伤科 [2]洛阳正骨研究所生物医学工程实验室 [3]上海中医药大学基础医学院分子生物学实验室

出　　处：《中华老年医学杂志》2002年第3期210-213,共4页Chinese Journal of Geriatrics

摘　　要：目的　研究卵巢切除术诱导的绝经后骨质疏松症大鼠模型股骨颈质量的力学表达与附着肌群肌强度的基因表达的变化 ,探讨股骨颈骨质疏松性骨折风险升高的肌肉 骨骼机制。　方法　2 0只 8月龄Wistar雌性大鼠随机分为对照组 10只 ,双侧卵巢假切除 ;模型组 10只为双侧卵巢切除。手术后第 180天 ,分别测定股骨颈质量的结构强度、结构硬度和结构韧性与附着股骨颈的外展肌群肌强度的重链肌球蛋白基因的表达。　结果　模型组的结构强度 ( 168 0 9± 2 5 2 0 )N、结构硬度( 682 3 0± 13 1 2 7)N/mm和结构韧性 ( 0 0 4± 0 0 7)J与对照组比较都显著下降 (P <0 0 5) ;附着的外展肌群肌强度的基因表达 ,对照组重链肌球蛋白ImRNA表达量高 ,模型组表达量低。　结论　股骨颈质量的低结构强度、低结构硬度与低结构韧性的力学表达导致股骨颈骨质疏松性骨折风险升高 ;附着肌群肌强度的重链肌球蛋白ImRNA低表达对于造成骨质量力学表达变化的骨重建高转换的调控机制具有重要的作用。提示降低股骨颈骨质疏松性骨折风险的临床干预策略必须考虑“筋、骨齐治”。Objective To study the mechanical characteristics of femoral neck bone quality and the myosin heavy chain Ⅰ(MHC Ⅰ) mRNA related to the attached muscle strength in postmenopausal osteoporotic rat model induced by ovariectomy, and to probe into the muscle bone mechanisms of the rising risk of osteoporotic fracture of the femoral neck. Methods Twenty 8 month old female Wistar rats were divided randomly into two groups: Sham operated control group (10 cases) and ovariectomy (OVX) group (10 cases). At the 180th day after surgery, the mechanical characteristics (max load, stiffness and energy) of the femoral neck bone quality based on material test and the gene expression (MHC Ⅰ mRNA) of the attached muscle strength based on RT PCR were examined. Results The mechanical characteristics including the max load, stiffness and energy in OVX group were reduced than that in the Sham operated control group ( P <0 05). The higher expression of the MHC Ⅰ mRNA in Sham operated control group was identified clearly, but that in OVX group was not clearly identified. Conclusions The lowering of the mechanical characteristics in bone quality can induce the rising risk of osteoporotic fracture of the femoral neck. The lower gene expression of the attached muscle strength is contributed to the regulation mechanism of high bone remodeling rate leading to the lower mechanical characteristics of bone quality. So 'bones and muscles together be treated' should be considered as the clinical strategy in reducing the osteoporotic fracture risk of the femoral neck.

关 键 词：卵巢切除 大鼠 股骨颈质量 力学特征 附着肌群肌强度 骨质疏松 基因表达 股骨颈骨折 

分 类 号：R-332[医药卫生]