解偶联蛋白-2 mRNA在LPS致热小鼠肝脏中的表达  

The Expression of Uncoupling Protein-2 mRNA in Livers of Feverish Mice Caused by Lipopolysaccharide

作　　者：宋阳[1] 马文樵 姜成钢[3] 冯甲棣[1] 

机构地区：[1]中国医科大学基础医学院体温研究室,辽宁沈阳110001 [2]江苏省泰州职业技术学校 [3]中国医科大学83期英文班

出　　处：《中国医科大学学报》2002年第3期177-179,共3页Journal of China Medical University

摘　　要：目的 :在细胞水平上精确定位解偶联蛋白 2 (uncouplingprotein 2 ,UCP2 )在肝脏组织中的表达 ,同时观察腹腔内注射细菌脂多糖 (lipopolysaccharide ,LPS)后不同时程UCP2 mRNA在肝脏中的表达 ,借此进一步探讨UCP2 在LPS引起的内毒素 (endotoxin ,ET)性发热中的作用。方法 :应用原位杂交 (in situhybridization ,ISH)技术和计算机图像分析技术检测能够反应小鼠肝脏中UCP2 mRNA表达的 3项重要参数值。结果 :LPS诱导肝细胞表达UCP2 mRNA ,使UCP2 mRNA在LPS致热小鼠肝脏中的表达显著高于同时期对照组的表达。结论 :在LPS引起的ET性发热中 ,LPS诱导肝细胞表达UCP2 mRNA ,使UCP2mRNA在肝脏中的表达增加。Objective: This study was designed to locate the expression of UCP 2 mRNA and to observe its expression in livers at different time points after intraperitoneal injection of lipopolysaccharide (LPS). Methods: Three important indices which can show the UCP 2 mRNA expression in livers of mice were measured by the method of in-situ hybridization and analyzed by using microscopic image quantitative analysis technique. Results: The LPS inducted the expression of UCP 2 mRNA in hepatocytes and improved the expression of UCP 2 mRNA in livers of feverish mice caused by LPS. The expression in feverish mice was much stronger than that of the control groups at the same time points. Conclusion: In endotoxic fever caused by LPS, LPS can induce the expression of UCP 2 mRNA in hepatocytes and improve its expression in livers of mice.

关 键 词：解偶联蛋白-2 MRNA LPS 致热小鼠 肝脏 脂多糖 原位杂交 

分 类 号：R575[医药卫生—消化系统]