p16和Rb在宫颈上皮内瘤样变及癌中的表达  

Expression of p16 and Rb proteins in cervical intraepithelial neoplasia and carcinoma

作　　者：王冰[1] 徐长福[1] 王晓丽[2] 莫立平[1] 李明众[2] 

机构地区：[1]西安交通大学医学院病理学教研室,陕西西安710061 [2]西安交通大学第一医院肿瘤放射治疗科,陕西西安710061

出　　处：《第四军医大学学报》2002年第11期1003-1005,共3页Journal of the Fourth Military Medical University

摘　　要：目的　探讨 p16和 Rb基因产物在宫颈癌中的表达 ,及与临床病理参数间的关系 .方法　应用免疫组化技术 (SP法 ) ,检测 p16 ,Rb基因在 10例正常宫颈组织、10例宫颈上皮内瘤样变 (CIN)及 5 9例宫颈鳞癌中的表达 .结果　 2 0 % CIN(2 / 10 )和 2 9%宫颈鳞癌 (17/ 5 9)中 ,p16免疫反应阴性 .p16蛋白表达缺失在不同年龄间有显著差异 ,而在肿瘤生长类型、临床分期及组织学分级各组间比较无显著差异 .宫颈癌中Rb表达阳性率为 88% (5 2 / 5 9) ,随组织学分级增高 ,Rb蛋白表达率升高 ,各组间比较有显著差异 . 6 6 %的宫颈癌两种蛋白共表达阳性 .结论　 p16失活参与部分宫颈癌的发生 .AIM To study the expression of p16 and Rb in cervical carcinoma and its relationship with clinicopathologic features. METHODS Using SP immunohistochemical technique, we examined the expression of p16 and Rb in 10 normal cervical tissues, 10 cervical intraepithelial neoplasias and59 invasive squamous cell carcinomas. RESULTS 20% CIN (2/10) and 29% cervical carcinomas (17/59) showed negative p16 staining. Abnormal p16 expression was related to age of patients. There were no statistically significant differences in the p16 status with respect to gross type, histologic differentiation and stage of the disease. Rb was expressed in 52 of 59 (88%) cases of cervical carcinomas. Rb expression was significantly correlated with histologic differentiation ( P < 0.005). 66% of specimens was immunoreactive for both Rb and p16. CONCLUSION Inactivation of p16 may play an important role in the genesis of human cervical carcinoma. Rb may be functionally inactivated in cervical cancer.

关 键 词：P16基因 RB基因 宫颈上皮内瘤样变 基因表达 宫颈癌 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]