人HL-60白血病细胞混合抗原肽诱导特异性免疫应答的研究  

Tumor antigen peptides from HL-60 cells induce specific immune response

作　　者：黄波[1] 冯作化[1] 张桂梅[1] 

机构地区：[1]华中科技大学同济医学院医学分子生物学研究室,武汉430030

出　　处：《中华血液学杂志》2002年第6期285-288,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金资助项目 (3 9970 3 2 2 );教育部跨世纪人才培养计划基金资助项目

摘　　要：目的　研究人HL 6 0白血病细胞混合抗原肽诱导特异性免疫应答的作用及其特异性。方法　用细胞冻融、加热沉淀及酸处理等方法除去HL 6 0细胞的核酸、脂质及大部分蛋白 ,制备混合抗原肽 ;体外将抗原肽与热休克蛋白 70 (Hsp70 )结合 ,以T细胞体外激活试验检测结合物对人外周血单个核细胞 (PBMNC)的刺激作用 ,并对激活的淋巴细胞进行传代培养 ,观察细胞的增殖 ;收集传代增殖的淋巴细胞 ,检测其对HL 6 0及K5 6 2白血病细胞的杀伤功能。结果　采用上述方法制备的抗原肽为混合肽 ,该混合肽经Hsp70提呈后 ,体外能激活PBMNC ,并使激活的细胞增殖 ,增殖的淋巴细胞可特异性地杀伤HL 6 0细胞 ,但对K5 6 2细胞无杀伤作用。结论　白血病细胞内含有可用生化方法制备的特异性抗原肽 ;利用Hsp70提呈此抗原肽可激活细胞毒性T淋巴细胞增殖 ,对白血病细胞产生特异性的杀伤作用 。Objective To prepare tumor antigen peptides from HL 60 cells and to induce specific immune response. Methods HL 60 antigen peptides were obtained using techniques including freezing and thawing,heat precipitation and acid precipitation. The stimulating effect of the in vitro Hsp70 binding HL 60 peptides on PBMC and the proliferation of stimulated PBMC were observed by T cell activation test. The cytotoxicity of proliferated PBMC is detected by incubating HL 60 cells or K562 cells with PBMC respectively. Results The obtained tumor antigen peptides were a peptides mixture. The mixed peptides could activate PBMC and cause PBMC proliferation in vitro after presented by Hsp70. The proliferated PBMC showed specific cytotoxicity to HL 60 cells but not to K562 cells. Conclusion The method for preparing of human leukemia tumor antigen peptides used in this paper is simple and easy; the obtained antigen peptides can induce specific immune response in vitro.

关 键 词：HL-60细胞系 白血病 抗原肽 特异性免疫应答 组织相容性抗原 

分 类 号：R733.7[医药卫生—肿瘤]