基底细胞痣综合征的牙源性角化囊肿中Ki-67表达的研究  

Ki-67 expression in nevoid basal cell carcinoma syndrome associated odontogenic keratocysts

作　　者：孙明磊[1] 廖小宜[1] 王炼[2] 沈翔 

机构地区：[1]四川大学华西口腔医学院口腔颌面外科,610041 [2]四川大学华西口腔医学院病理科,610041 [3]南京市口腔医院正畸科

出　　处：《实用口腔医学杂志》2002年第3期220-222,共3页Journal of Practical Stomatology

摘　　要：目的 :探讨基底细胞痣综合征的牙源性角化囊肿细胞增殖活性与临床生物学行为的关系。方法 :利用Ki 67单克隆抗体 ,免疫组化方法 (LSAB法 )检测基底细胞痣综合征的牙源性角化囊肿和非综合征的牙源性角化囊肿中Ki 67表达情况。结果 :Ki 67在基底细胞痣综合征的牙源性角化囊肿衬里上皮中的表达主要位于基底上层 ,且明显高于单发和复发牙源性角化囊肿。结论 :基底细胞痣综合征的牙源性角化囊肿较非综合征的角化囊肿具有更高的细胞增殖活性 。Objective: To investigate the proliferation of three subtypes of odontogenic keratocysts(OKC), namely simple(not recurrent),recurrent and basal cell naevus syndrome(BCNS) associated lesions. Methods: Ki 67 expression was studied in 34 odontogenic keratocysts(simple, n =10;recurrent, n =12;syndrome, n =12)by biotin streptavidin method using Ki 67 monoclonal antibody after microwave treatment. Ki 67 positive cells were counted manually and related to the area of epithelial lining as determined by computer image analyzer. Results: Ki 67 positive cells per mm 2 in simple odontogenic keratocysts ①, recurrent odontogenic keratocysts ② and BCNS ③ were 1 812.29±606.47, 2 393.88 ±997.08 and 3 983.17±858.92 respectively(① or ② vs ③ P <0.01; ① vs ② P >0.05). Conclusion: The basal cell naevus syndrome associated OKC has a higher rate of epithelial proliferation than non syndrome OKC, the increased epithelial proliferation is correlated with increased recurrence potential. Ki 67 may be an alternative method to differentiate syndrome and non syndrome OKC.

关 键 词：基底细胞痣综合征 牙源性角化囊肿 KI-67 细胞增殖 

分 类 号：R780.2[医药卫生—口腔医学]