红藻氨酸诱发大鼠复杂部分性癫痫发作后延髓内脏带内FOS蛋白、GFAP与TH的共同表达  

Expression of FOS protein,GFAP and TH in rat medullary visceral zone after complex partial seizure induced by kainate acid

作　　者：田国红[1] 段丽[1] 饶志仁[1] 黄远桂[2] 徐燕[2] 

机构地区：[1]第四军医大学神经科学研究所 [2]西京医院神经内科,西安710032

出　　处：《解剖学杂志》2002年第3期240-244,共5页Chinese Journal of Anatomy

摘　　要：目的:观察大鼠癫痫发作后延髓内脏带内神经元和星形胶质细胞活性变化的时空效应及与儿茶酚胺类递质的关系。方法:以红藻氨酸诱导大鼠复杂部分性发作为癫痫模型,利用免疫组织化学三重染色技术,显示癫痫发作后30min至6h不同时点延髓内脏带区域同一部位FOS蛋白与胶质原纤维酸性蛋白（GFAP）及酪氨酸羟化酶（TH）的表达变化与相互关系及分布规律。结果:癫痫发作后延髓内脏带内c-fos表达与GFAP阳性细胞分布基本一致,且癫痫诱发后30min GFAP开始增多,1h达高峰;FOS阳性产物1h开始增多,3h达高峰;部分FOS阳性神经元为TH阳性,周围有GFAP免疫反应产物包绕。结论,在癫痫病理状态下,延髓区域星形胶质细胞的反应略早于神经元,二者不但分布上具有一一对应的关系,它们之间可能存在着复杂的信息通讯,形成神经元-胶质细胞复合体,共同对各种刺激做出反应。bjective:To investigate the temporal and spatial activity change of the neurons and astrocytes in the medullary visceral zone (MVZ) after seizures,and to reveal the relationship of activated neurons with the catecholaminergic transmitter. Methods; Immunohistochemical triple staining technique was used to show the expression and distribution pattern of FOS-protein,GFAP and TH at different time points in the rat brain after complex partial seizures were kindled by kainate acid. Results;The distribution of FOS like positive neurons was fundamentally similar to the pattern of GFAP positive cells in the MVZ. The expression of GFAP began to increase 30 min after seizure induction, and reached maximum at 1 h; while FOS positive neurons began to appear at 1 h,peaked at 3 h. Some double labeled neurons of FOS and TH were encapsuled by concentrated GFAP positive fibers. Conclusion: Under epileptic condition, the reaction of astrocrytes were earlier than that of the neurons in the MVZ. The pattern of distribution of them was similar and there may be complicate information communication,and then through a functional complex reacting to various stimuli together.

关 键 词：红藻氨酸 大鼠 复杂部分性癫痫 发作后 延髓内脏带 FOS蛋白 GFAP TH 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]