氧苯胂对人皮肤成纤维细胞缝隙连接通讯及DNA损伤的影响  

Study of the inhibitory effect of PhAsO on gap junctional communication and its damages to cellular DNA of human skin fibroblast cells

作　　者：邓芙蓉[1] 金昱[1] 王慧[1] 李新华[1] 郭新彪[1] 

机构地区：[1]北京大学公共卫生学院,北京100083

出　　处：《卫生研究》2002年第3期151-153,共3页Journal of Hygiene Research

基　　金：美国中华医学基金人材专项基金项目资助课题;(ORF980 4 )

摘　　要：分别采用细胞划痕染料标记示踪技术和单细胞凝胶电泳技术 ,研究了氧苯胂对人皮肤成纤维细胞缝隙连接通讯的影响及DNA的损伤作用。研究结果显示 ,氧苯胂可显著抑制细胞缝隙连接通讯 ,其作用有明显的剂量 -反应关系 (P <0 0 1) ,在浓度为 10 0nmol L时抑制作用最强 ;氧苯胂对细胞DNA的损伤作用不明显 ,损伤细胞以尾长小于头长的轻微型DNA断裂为主。以上研究结果提示 ,氧苯胂的遗传毒性较弱 ,而其非遗传毒性相对较强 。In order to investigate the toxicity of organic arsenic as well as the mechanism of arsenic carcinogenesis, the GJIC between human skin fibroblast cells is detected by the scrape loading /dye transfer assay and single cell gel electrophoresis(SCGE) assay is used to detect the DNA strand breaks in human skin fibroblast cells exposed to PhAsO. The results show that gap junctional intercellular communication between human skin fibroblast cells is inhibited by PhAsO significantly with a obvious dose dependent relationship( P <0 01), suggesting that PhAsO may act as a tumour promoter. PhAsO could induce DNA strand breaks, but the dose dependent relationship is not observed. The authors suggest that PhAsO may have tumor promoting activity, and further studies are needed.

关 键 词：氧苯胂 皮肤 成纤维细胞 缝隙连接通讯 DNA损伤 致癌作用 

分 类 号：R730.231.1[医药卫生—肿瘤]