用小鼠胚胎肢体器官培养研究锌对骨发育的影响  

Study of the effects of zinc on bone development applying fetal mouse limbs culture

作　　者：余增丽[1] 李云[1] 段广才[1] 

机构地区：[1]四川大学华西公共卫生学院,成都610041

出　　处：《卫生研究》2002年第3期166-168,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目 (No .3960 0 1 2 2 )

摘　　要：用自制浸浴式一次通气旋转装置对孕龄 16d小鼠胚胎前肢进行培养 ,采用组织学分析及检测骨组织中AKP活性的方法研究锌对骨发育的影响。结果表明 ,与对照组相比 ,锌缺乏组和过量补锌组 (终浓度含锌 12 0 μmol L)的骨组织发育延迟 ,骨组织中AKP活力降低 ;而适量补锌组 (终浓度含锌 4 5 μmol L及 70 μmol L)则促进成骨细胞向骨细胞增殖、分化 ,增加类骨质的分泌与合成 ,促进类骨质向骨质转化 。The effects of zinc on bone metabolism is explored. A home made rotating apparatus is used to cultivate fetal mouse front limbs at 16-day gestational age. The activity of alkaline phosphatase(AKP) and histological silce are used to analyze the effects of zinc on bone metabolism. Compared with the control group, the zinc‐deprived group and Zn 2+ 120μmol/L group have a lower alkaline phosphatase (AKP) activity and the contracted chromatin, membrane rupture and matrix loss are observed in bone cells. The zinc adequate group(Zn 2+ concentration are 45μmol/L and Zn 2+ 70μmol/L respectively) has a higher alkaline phosphatase(AKP) activity, more active bone generation, deeper stain of bone matrix and increased bone trabecula. It is concluded that zinc could affected the bone metabolism and growth; both zinc deficiency and zinc excess could alter growth and bone metabolism.

关 键 词：小鼠 胚胎 肢体器官 培养 锌 骨发育 

分 类 号：Q593.6[生物学—生物化学] R151.3[医药卫生—营养与食品卫生学]