细胞因子等对人原代培养肝细胞细胞间黏附分子-1表达的调控  

Study on ICAM-1 expression of primary cultured human hepatocyte regulated by cytokines and dexamethasone

作　　者：张绪清[1] 张瑞[1] 顾长海[1] 王宇明[1] 

机构地区：[1]第三军医大学西南医院感染病科,重庆400038

出　　处：《中华传染病杂志》2002年第3期172-174,共3页Chinese Journal of Infectious Diseases

摘　　要：目的　研究IFN γ、TNF α、IL 1和地塞米松对人原代培养肝细胞细胞间黏附分子 1(ICAM 1)表达的影响。方法　用IFN γ、TNF α和IL 1在体外诱导人原代培养肝细胞表达ICAM 1,并用细胞ELISA检测其ICAM 1表达水平 ;在用细胞因子刺激前先加入地塞米松 ,观察地塞米松对人肝细胞ICAM 1表达的影响。结果　未经刺激的人原代培养肝细胞ICAM 1表达水平很低 ;TNF α、IL 1、IFN γ刺激后 ,人原代培养肝细胞ICAM 1表达均明显增强 ,其表达水平与细胞因子刺激之间存在一定的剂量和时间依赖关系 ;地塞米松能部分抑制TNF α、IL 1、IFN γ所诱导的人原代培养肝细胞ICAM 1增强表达 ,其抑制率分别为 (4 0 .3± 5 .9) %、(3 8.1± 4.8) %和 (3 7.6± 6.7) %。结论　IFN γ、TNF α和IL 1能诱导人原代培养肝细胞ICAM 1增强表达 ,而地塞米松能抑制其表达。Objective To study the effects of IFN γ,TNF α,IL 1 and dexamethasone on the expression of intercellular adhesion molecule 1(ICAM 1) of primary cultured human hepatocyte. Methods ICAM 1 expression of primary cultured human hepatocyte was induced in vitro by IFN γ,TNF α and IL 1, measuring by cellular enzyme linked immunosorbent assay(ELISA). The effect of dexamethasone on human hepatocyte ICAM 1 expression was observed by adding dexamethasone into the cultured human hepatocytes prior to cytokine stimulation. Results The levels of ICAM 1 expression were significantly lower in unstimulated human hepatocytes than in human hepatocytes induced by IFN γ,TNF α and IL 1. There were relations among the levels of ICAM 1 expression and cytokine concentration as well as induced period of time. Dexamethasone could partly inhibit the ICAM 1 expression on human hepatocytes induced by TNF α,IL 1 and IFN γ.The inhibition rates were 40.3%±5.9%,38.1%±4.8%, and 37.6%±6.7%, respectively.Conclusions Enhanced ICAM 1 expression of primary cultured human hepatocyte may be induced in vitro by IFN γ,TNF α and IL 1, which may be partly inhibited by dexamethasone.

关 键 词：细胞因子 人原代培养 肝细胞 细胞间黏附分子-1 自身免疫性肝炎 炎症反应 地塞米松 

分 类 号：R575[医药卫生—消化系统]