血管内抑素转基因双歧杆菌对人胃癌影响的初步病理观察  

The Effect of Endostatin Transfected Bifdobacterium Oral Powder Preparation (ETB-2) on Human Gastric Carcinoma

作　　者：王英[1] 王晓熙[1] 沈宾鸿[1] 丁艾妮[1] 倪海东[1] 王喜安[1] 金冠球[1] 

机构地区：[1]解放军第四一一医院病理科,上海200081

出　　处：《海军医学杂志》2002年第2期114-117,共4页Journal of Navy Medicine

摘　　要：目的 :观察血管内抑素转基因双歧杆菌口服冻干粉 (ETB 2 )经胃癌患者服用后病理组织学变化和微血管密度(MVD)值、增殖细胞核抗原 (PCNA)活性表达 ;评价ETB 2抑制胃癌微血管生长的效果。方法 :采用免疫组织化学和常规染色方法对 2 0例晚期胃癌进行病理检测 ,结合应用图像分析系统计数分析 ,重点观测服药后癌组织的特异性病理变化和MVD值、PCNA活性表达。结果 :2 0例服药组与 2 3例未服药组比较 ,癌组织显现出具特异性的病理组织学改变 ,主要有 :①坏死灶形成 ;②微脓疡形成 ;③纤维组织增生。免疫组织化学检测结果MVD值显著减少 (P <0 .0 5 )、PCNA活性极显著降低 (P <0 .0 1)。结论 :ETB 2口服制剂可抑制晚期胃癌组织血管新生 ,致癌组织发生显著的缺血性改变 ,并降低癌细胞增殖活性 。Objective: To investigate the effect of endostatin transfected bifdobacterium oral powder preparation (ETB 2) on the pathology, microvessel density (MVD) and proliferating cell nuclear antigen (PCNA) of human gastric carcinoma. Methods: The ETB 2 group consisted of 20 cases with gastric carcinoma; the negative group included 23 cases; the control group included 20 cases from normal tissues near the cancer. Using immunohistochemistry and image analysis system, changes in histopathology, MVD and PCNA were observed. Results: In the ETB 2 group, histopathologic changes found included the formation of necrosis and microabscess, and the proliferation of fibrous tissue. The MVD and PCNA in the ETB 2 group were reduced significantly, compared with those in the control group. Conclusion: ETB 2 have antiangiogenetic effect on gastric carcinoma, inducing ischemia of cancer tissue and inhibiting proliferation of cancer.

关 键 词：胃肿瘤 血管内抑素 双歧杆菌 

分 类 号：R735.2[医药卫生—肿瘤]