血管内皮细胞生长因子抑制剂对成骨肉瘤生长的影响  

Anti-VEGF Monoclonal Antibody Inhibit the Growth of Osteosarcoma

作　　者：蔡槱伯[1] 张伟[1] 

机构地区：[1]北京积水潭医院骨肿瘤科

出　　处：《首都医科大学学报》2002年第2期153-155,共3页Journal of Capital Medical University

摘　　要：通过动物接种方法研究血管内皮细胞生长因子 (VEGF)抗体对骨肉瘤生长的抑制作用。将OS 732骨肉瘤细胞以 1 0 70 / (mL·只 )的剂量接种于BALB/C型裸鼠腋窝部皮下 ,并分成 4组 :A组于接种次日于瘤体局部注射VEGF抗体 ,B组于接种后 2周瘤体局部注射抗体 ,C组于接种次日于瘤体局部注射PBS ,D组不注射抗体。VEGF注射剂量为 2 0 0 μg/ (只·次 ) ,每周 3次 ,共 3周。 4周后处死A、C2组裸鼠 ,6周后处死B、D 2组裸鼠。每组均取出瘤组织作病理切片。计算肿瘤体积及肿瘤内微血管密度 ,并对结果进行分析。结果显示 ,A组的肿瘤体积小于B、C、D 3组 ,在接种后 2 5d与D组有统计学差异 (P <0 .0 5 ) ;B、C、D 3组间无统计学差异。 4组肿瘤内微血管密度的比较 ,无统计学差异。提示 ,VEGF抗体对骨肉瘤早期的生长有明显的抑制作用 ,可作为转移病灶的预防及治疗方法之一。In this study, the availability of specific monoclonal antibodies capable of blocking VEGF-induced angiogenesis in vivo allowed us to test the hypothesis directly. The aim of our study was to observe the inhibition of growth of osteosarcoma by anti-VEGF monoclonal antibody through animal experiment. We also investigate the effect of anti-VEGF antibody on microvessel density in tumors through histological method. Twenty-three female BALB/C nude mice were used in this study. OS-732 osteosarcoma cells were injected into the armpit subcutaneous part (1 070/mL for each mouse). The nude mice are divided into four groups randomly (A, B, C, and D). The anti-VEGF monoclonal antibody was injected into the six mice of group A at the next day of the osteosarcoma cell-injection. Two weeks later, the antibody was injected into the six mice of group B. PBS was injected into the six of group C and nothing to the five of group D at the same time with group A. The single application of the antibody was 200 μg for each mouse three times weekly for three weeks. The mice of group A and C were killed four weeks later and group B and D six weeks later. Every mouse was anatomized and the tumor was taken out for a pathological slice. The tumor size and counted the microvessel density were meesured. Comparisons of the results with and without the treatment were performed using a Student T-test. A value of P< 0.05 was considered significant. The tumor growth was significantly inhibited only when the anti-VEGF antibody administration was started immediately after tumor cell inoculation (group A) but not when such treatment was initiated two weeks later (group B), when the tumors were already established. When comparing the microvessel density, there were no significant differences between the four groups. In summary, our results demonstrated, for the first time, that the incipient growth of osteosarcoma could be inhibited significantly by anti-VEGF antibody. The anti-VEGF antibody may be useful for the prevention and clinical treatment

关 键 词：血管内皮细胞生长因子 成骨肉瘤 VEGF抗体 动物实验 微血管密度 

分 类 号：R738.7[医药卫生—肿瘤]