温度及药物诱导肿瘤细胞凋亡的实验观察  

Experimentation on Induction of Tumor Cell Apoptosis by Temperature and Anti-tumor Medicine

作　　者：李莉[1] 张珈敏[2] 胡远扬[2] 冯英[3] 屈松生[3] 

机构地区：[1]武汉大学生命科学院结构病毒室武汉大学化学与分子科学学院,武汉430072 [2]武汉大学生命科学院结构病毒室 [3]武汉大学化学与分子科学学院,武汉430072

出　　处：《高等学校化学学报》2002年第7期1379-1382,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 9773 0 3 3 )

摘　　要：将微量热法和透射电镜技术相结合 ,研究了温度对人卵巢癌细胞代谢的影响 ,不同温度下癌细胞超微结构的变化和加抗肿瘤药物 (嘧福绿 )的协同作用 .从宏观到微观 ,深入探讨了热疗与化疗协同作用对肿瘤细胞杀伤作用的机理 .实验结果表明 ,在高温下细胞代谢降低 ,肿瘤细胞的形态发生一系列恶性变化 ,且高热可能诱导肿瘤细胞的凋亡 ;热疗加化疗的协同作用则能加速肿瘤细胞的凋亡 ,使细胞的能量代谢更低 ,随着作用时间的延长 。Techniques from microcalorimetry and transmission electron microscopy(TEM) were employed to investigate the metabolic effects and the ultrastructural changes of human body carcinoma of ovary cell line(COC 1) at different temperatures with and without synergetic effects of anti tumor medicine(HCFU). The mechanism of hyperthemia and chemotherapy in the treatment of the tumor cells was explored at both macroscopic and microscopic levels. The results showed that the metabolic activity of the tumor cells was reduced at high temperature and the cell morphology changed malignantly. High temperature might induce apoptosis of the tumor cells; and the combined effects of hyperthemia and chemotherapy could speed up the apoptosis and reduce the metabolic power of the tumor cells. A sustained longer time action could lead to ultimate death of the tumor cells.

关 键 词：温度 药物 细胞凋亡 微理热法 透射电镜 肿瘤细胞 高热疗法 化学治疗 协同作用 

分 类 号：R730.53[医药卫生—肿瘤] R730.59[医药卫生—临床医学]