金电极表面植物原生质体的固定化  

Immobilization of Plant Protoplast on Gold Electrode Surface

作　　者：张红平[1] 祁玉兰[1] 严曼明[1] 韦毅[2] 林仲华[3] 江志裕[1] 

机构地区：[1]复旦大学化学系 [2]复旦大学生命科学院,上海200433 [3]厦门大学化学系

出　　处：《高等学校化学学报》2002年第7期1392-1394,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 9773 0 0 9和 2 983 3 0 60 )

摘　　要：Barley protoplast was immobilized on poly(dially dimethylammonium) (PDADMAC) modified gold electrode via the electrostatic attraction between the positively charged amine groups of PDADMAC and the negatively charged barley protoplast. STM image reveals that PDADMAC/Au(111) surface displays a nano organized dot(4-6 nm) array structure. The processes of the PDADMAC adsorption and of the barley protoplast immobilization were monitored from the in situ QCM frequency change. The effects of the adsorbed mass and of the density and viscosity of medium on the frequency change of QCM were discussed, respectively. The QCM result shows that the thickness of the adsorbed PDADMAC layer is 2.4 nm.Barley protoplast was immobilized on poly(dially dimethylammonium) (PDADMAC) modified gold electrode via the electrostatic attraction between the positively charged amine groups of PDADMAC and the negatively charged barley protoplast. STM image reveals that PDADMAC/Au(111) surface displays a nano organized dot(4-6 nm) array structure. The processes of the PDADMAC adsorption and of the barley protoplast immobilization were monitored from the in situ QCM frequency change. The effects of the adsorbed mass and of the density and viscosity of medium on the frequency change of QCM were discussed, respectively. The QCM result shows that the thickness of the adsorbed PDADMAC layer is 2.4 nm.

关 键 词：金电极 植物原生质体 聚电解质 大麦细胞 表面固定化 STM QCM 生物活细胞 

分 类 号：Q814.2[生物学—生物工程]