人乳铁蛋白在原核中的融合表达  

Cloning and fusion expression of human lactoferrin protein gene in prokaryotic expression vector

作　　者：李树芹[1] 章立群[1] 孙雪梅 贾士乾[1] 周伟明[1] 张建华 吕加平[1] 张列兵[1] 

机构地区：[1]北京三元食品股份有限公司,北京102206

出　　处：《中国乳品工业》2002年第3期3-5,共3页China Dairy Industry

摘　　要：设计了特异引物,通过PCR扩增人乳铁蛋白(HLF)基因,将扩增的DNA片段重组到原核表达载体pGEX-4T3中,重组体转化大肠杆菌BL21菌株,经IPTG诱导后,得到了高效融合表达。分析结果表明,获得了特异表达的乳铁蛋白。By designing special,primers,human lactoferrin protein gene was amplified by PCR and cloned into pGEX-4T3 vector,then transformed it into escherichia coli BL21.Induced by IPTG,the fusion protein was expressed specially.The result of western blot analysis indicated that the fusion protein was GST-human lactoferrin protein.

关 键 词：人乳 铁蛋白 PCR 融合表达 大肠杆菌BL21菌株 

分 类 号：Q592.6[生物学—生物化学] TS252.1[轻工技术与工程—农产品加工及贮藏工程]