外周血中白蛋白mRNA作为肝癌微小转移标志物的进一步研究  

Detection of albumin mRNA in the peripheral blood of patients with HCC and other liver diseases

作　　者：智慧[1] 邓庆丽[2] 詹俊[3] 周雨迁[2] 邵静[2] 黄志明[2] 

机构地区：[1]广州中山大学孙逸仙纪念医院超声科,510120 [2]广州中山大学孙逸仙纪念医院医学研究中心,510120 [3]广州中山大学孙逸仙纪念医院消化内科,510120

出　　处：《肝脏》2002年第2期95-97,共3页Chinese Hepatology

基　　金：97.3分课题资助项目 (G1 9990 541 0 5)

摘　　要：目的　检测不同肝脏疾病患者的外周血中白蛋mRNA(ALBmRNA) ,探讨外周血中ALBmRNA作为肝癌微小转移标志物的特异性。方法　分离原发性肝癌患者以及肝硬化、急慢性肝炎、肝转移癌、肝脏良性肿瘤患者和健康志愿者的外周血单个核细胞 ,提取单个核细胞的总RNA ,进行RT PCR ,将RT PCR产物再进行Southern杂交。结果　原发性肝癌患者组外周血中ALBmRNA的检出率为 44 .8% ,对照组检出率为 2 9.2 % ,两者之间的差异无统计学意义 (P≥ 0 .0 5 )。Objective To study the specificity of albumin mRNA (ALB mRNA) positive cells in the peripheral blood as an indicator for circulating malignant hepatocyte. Methods Using RT PCR/Southern blot to detect the ALB mRNA in the peripheral blood from 29 patients with hepatocellular carcinoma (HCC) and 41 cases without HCC (8 cases of cirrhosis, 4 cases of acute hepatitis, 15 cases of chronic hepatitis, 2 cases of hepatic angioma, 2 cases of non HCC hepatic tumor and 10 normal voluteers). Results In the patients with HCC, the ALB mRNA was found in 13 out of 29 cases (44.8%). In the control group, the ALB mRNA was found in 12 out of 41 cases (29.2%). There was no significant difference between the ALB mRNA positive rates of these two groups ( P ≥0.05). Conclusion As an index of liver cancer cell in peripheral blood, ALB mRNA is non specific.

关 键 词：肝细胞癌 微小转移 蛋白MRNA 逆转录聚合酶链反应 SOUTHERN杂交 

分 类 号：R735.7[医药卫生—肿瘤]