HCPT联合Ver防治实验性增生性玻璃体视网膜病变的初步研究  

Prevention of experimental proliferative vitreoretinopathy by hydroxycamptothecine and Verapamil

作　　者：毕燕龙 荣翱[2] 胡桃舫[2] 王惠英[2] 李锦文[2] 

机构地区：[1]上海铁路中心医院眼科,上海市200072 [2]同济大学附属同济医院眼科,200065

出　　处：《眼科新进展》2002年第3期166-169,共4页Recent Advances in Ophthalmology

基　　金：铁道部专项基金项目(编号 :TDB;视网膜色素上皮细胞移植方式及移植后视细胞凋亡的研究 )~~

摘　　要：目的　观察羟基喜树碱 (hydroxycom ptothecine,HCPT)联合 Ver对实验性增殖性玻璃体视网膜病变 (proliferative vitreoretinopathy,PVR)的防治作用。方法　以原代培养成纤维细胞 (fibroblast,FB)每眼 10 0 0 0 0个经兔眼睫状体扁平部注射到玻璃体腔内 ,制作成兔 PVR模型 ,共 12只兔 12眼。随机分成药物治疗组和生理盐水对照组 (每组 6只 ) ,治疗组以 5 m g· L- 1 HCPT联合 5 mg· L- 1 Ver进行每 3天 1次共 15 d的玻璃体腔注射治疗。对照组以同法注入生理盐水。以裂隙灯显微镜、直接检眼镜、螺旋 CT进行随访观察。结果　药物治疗组无论在玻璃体混浊程度进展上、在视网膜血管扩张程度上以及牵拉性视网膜脱离 (tractional retinal detachment,TRD)发生发展上都较对照组缓和。进行此浓度药物治疗后未见任何可见的眼部毒副作用。螺旋 CT可以监测到治疗组 PVR较对照组PVR在增殖膜密度上差异有显著性。结论　 HCPT联合 Ver经玻璃体腔内注射途径可以有效防治实验性兔眼 PVR。螺旋 CT可对兔眼 PVR进行定性。Objective To investigate the prevention of experimental proliferative vitreoretinopathy (PVR) by hydroxycamptothecine (HCPT) and Verapamil (Ver).Methods The primary cultured rabbit's fibroblast (100 000 per eye) were injected through pars plana into rabbits' vitreous to produce PVR models. Twelve rabbits with 12 eyes were studied and the experimental and the control groups contained 6 eyes respectively. 5mg·L -1 HCPT and 5mg·L -1 Ver were injected into PVR eyes of experimental group every 3 days till to 15 days , the control group's eyes were injected with saline with same methods in same period. Slit lamp microscope, direct ophthalmoscope, spiral CT were used as follow up observation methods. Results The experimental group's eyes had obviously treated function in the progress of vitreous opacity and the dilataltion of retinal vessals and the occurrence of tractional retinal detachment. Position and ill effects could not be detected. With spiral CT, we could monitor that there were obvious differences between the proliferative membrane of experimental and control groups.Conclusion Injection of HCPT combined with Verapamil into vitreous directly can effectively prevent and treat experimental rabbits' PVR. Spiral CT can analysis rabbits' PVR quantitatively and qualitatively.

关 键 词：HCPT Ver 羟基喜树碱 维拉帕米 视网膜病变 螺旋CT PVR 动脉模型 

分 类 号：R774.1[医药卫生—眼科] R776.4[医药卫生—临床医学]