中华眼镜蛇毒血清抑制HL60细胞增殖及诱导凋亡的实验研究  

Inhibition of proliferation and apoptosis in HL60 cells induced by cobra venom serum

作　　者：罗英儒[1] 叶春玲[1] 任先达[1] 李红良[1] 钟玲[1] 

机构地区：[1]暨南大学药学院药理学教研室,广州510632

出　　处：《中国药理学通报》2002年第3期291-293,共3页Chinese Pharmacological Bulletin

基　　金：广东省科委博士启动基金资助课题 No 9740 2 6

摘　　要：目的　探讨中华眼镜蛇毒血清体外对白血病细胞系(HL6 0 )的抑制作用及其机制。方法　以人粒细胞白血病细胞系 (HL6 0 )为靶细胞 ,观察口服中华眼镜蛇毒血清对肿瘤细胞增殖的影响 ,并采用DNA片断凝胶电泳、流式细胞仪分析药物体外诱导细胞凋亡情况。结果　中华眼镜蛇毒血清在体外显著抑制HL6 0细胞的生长 ,其抑制作用随着剂量的加大而增强 ,高剂量 (7 5g·L-1)的抑制效果相近于阿霉素(5mg·L-1) ,和空白对照组比较差异有显著性。经DNA电泳、流式细胞仪分析显示中华眼镜蛇毒血清与HL6 0细胞共同培养可诱导其发生细胞凋亡。结论　中华眼镜蛇毒血清体外能显著抑制HL6 0细胞的生长 ,此作用可能与诱导白血病细胞发生凋亡有密切关系。AIM To explore the effects and mechanism of cobra venom serum on the proliferation in HL60 cells. METHODS Established the HL60 cells as a target to study the growth feature by the action of cobra venom serum.The agarose gel electrophoresis and flow cytometry analysis were used to demonstrate apoptosis. RESULTS Compared with the control group, the cells were inhibited significantly by the action of cobra venom serum.A characteristic DNA 'ladder' was detected by using agarose gel electrophoresis. By flow cytometry analysis,it was proved that most apoptosis of HL60 cells occurred when cultured with cobra venom serum. CONCLUSION Cobra venom serum inhibited the HL60 cells in vitro , which was related to apoptosis. This may introduce a new way to the treatment of leukemia.

关 键 词：中华眼镜蛇毒 白血病 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤] R996.3[医药卫生—临床医学]