卡托普利和阿司匹林相互作用对培养乳鼠心肌细胞损伤的影响及其机制探讨  

Effects and mechanism of interactions between captopril and aspirin on injured myocardial cells from neonate rats

作　　者：吕雄文[1] 李俊[1] 金涌[1] 李常玉[1] 马传庚[1] 

机构地区：[1]安徽医科大学临床药理研究所,合肥230032

出　　处：《中国药理学通报》2002年第3期308-311,共4页Chinese Pharmacological Bulletin

摘　　要：目的　观察卡托普利和阿司匹林相互作用对培养乳鼠心肌细胞损伤的影响及其机制。方法　建立培养乳鼠心肌细胞损伤模型 ,分别采用酶联法及Griess试剂法测定培养液中乳酸脱氢酶和一氧化氮活性 ,采用Fura 2负载荧光技术检测胞内游离钙水平。结果　与模型组相比 ,卡托普利和阿司匹林均显著减少培养介质中LDH水平 ,显著增加NO生成 ,但二者联用存在显著的相互作用 ,且表现为拮抗效应。卡托普利显著减少胞内游离钙浓度 ,而阿司匹林单用及合用卡托普利均明显增加胞内游离钙浓度。结论　卡托普利和阿司匹林联用时对培养乳鼠心肌细胞损伤存在显著的相互作用 。AIM To observe the effects of interactions between captopril and aspirin on injured myocardial cells from neonate rats and its mechanism. METHODS The injury model of cultured neonatal rat myocardial cells was developed. The activity of lactate dehydrogenase (LDH) and the level of nitric oxide (NO) were measured by LDH kits and Griess reagent respectively, the intracellular free calcium concentrations were measured with Fura 2/AM. RESULTS Both captopril and aspirin could reduce obviously the activity of LDH and increase the level of NO in medium, but their combination produced significant antagonistic interactions. Captopril reduced obviously the intracellular free calcium concentrations in the injury model, but aspirin alone or in combination with captopril significantly increased it. CONCLUSION Captopril and aspirin combination produced significant antagonistic interactions, the effects may be related to its actions of reducing the production of NO and increasing the intracellular free calcium concentrations.

关 键 词：卡托普利 阿司匹林 心肌细胞培养 乳酸脱氢酶 一氧化氮 钙 

分 类 号：R－332[医药卫生] R322.11