环孢霉素A逆转Pgp多药耐药机理的研究  

Mechanism of Cyclosporin A on Reversal of the P-glycoprotein-mediated Multidrug-resistance

作　　者：龚玉萍[1] 刘霆[1] 贾永前[1] 邓承祺[1] 欧阳仁荣[2] 

机构地区：[1]四川大学华西医院血液科,成都610041 [2]上海第二医科大学附属仁济医院血液科

出　　处：《华西医科大学学报》2002年第3期394-396,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金资助 (批准号 30 10 0 0 74)

摘　　要：目的　探讨环孢霉素 A (Cs A)逆转糖蛋白 Pgp耐药细胞株 K5 6 2 /ADR耐药的机理。方法　采用RT- PCR、荧光法、MTT和共聚焦激光扫描显微镜等方法 ,研究 Cs A对柔红霉素 (DNR)在 Pgp耐药细胞株 K5 6 2 /ADR蓄积、毒性及分布的影响。结果　 Cs A可明显增加 DNR在耐药细胞内的蓄积 ,恢复 DNR在耐药细胞核、浆的弥漫分布 ,提高耐药细胞对 DNR的敏感性。结论　 Cs A通过增加耐药细胞内化疗药物含量和逆转化疗药物异常分布的作用 ,恢复耐药细胞对化疗药物的敏感性。Objective To inquire about the mechanism of cyclosporin A (CsA) on reversal of the P-glycoprotein-mediated multidrug-resistance. Methods The effect of cyclosporin A on the modification of daunorubicin (DNR) accumulation, cytotoxicity, distribution in the resistant K562/ADR was examined by RT-PCR, confocal scanning laser microscopy(CSLM), fluorometry and MTT methods. Results It was found that CsA can obviously increase DNR accumulation, restore DNR diffuse cytoplasmic and nuclear fluorescence distribution, and increase the sensitivity to DNR in the resistant cell line. Conclusion Cyclosporin A reverses multidrug-resistance by increasing accumulation and altering subcellular distribution of DNR in resistant cell line.

关 键 词：环孢霉素A 多药耐药 糖蛋白Pgp 逆转 

分 类 号：R730.5[医药卫生—肿瘤]