预防性治疗急性实验性自身免疫性脑脊髓炎的实验研究  

Experimental Study on Preventive Inhibition of Acute Experimental Autoimmune Encephalomyelitis

作　　者：周红雨[1] 董为伟[2] 

机构地区：[1]四川大学华西医院神经内科,成都610041 [2]重庆医科大学神经病学研究所

出　　处：《华西医科大学学报》2002年第3期404-406,436,共4页Journal of West China University of Medical Sciences

摘　　要：目的　观察己酮可可碱及溴隐亭能否抑制急性实验性自身免疫性脑脊髓炎 (EAE)模型的发病 ,以及对血清 TNF、IL- 6活性及 PRL 水平的影响 ,为临床应用提供实验依据。方法　将 EAE模型豚鼠随机分成两组 ,分别给予治疗组肌注己酮可可碱 (30 mg/ kg· d)或口服溴隐亭 (5 mg/ kg· d) ,对照组肌注等容量的无菌生理盐水或口服等容量 5 %葡萄糖液。观察动物 EAE的发病数、临床评分、病灶计数以及检测各组动物血清 TNF、IL- 6活性及血清 PRL 水平。结果　己酮可可碱及溴隐亭治疗组豚鼠发病数较对照组减少 ,临床表现及病理变化明显减轻 ,血清 TNF、IL- 6活性及 PRL 水平较对照组明显降低。结论　己酮可可碱可能通过抑制 TNF释放及其活性的表达 ,而溴隐亭可能通过抑制 PRL 释放 ,从而抑制 EAE的发病 ,可尝试将该药物用于临床 MS的急性发作或复发的治疗。Objective To determine whether Pentoxifylline and Bromocriptine reduce both the severity and incidence of acute experimental autoimmune encephalomyelitis (EAE) by inhibiting the activity of serum TNF, IL-6 and secretion of prolactin, thus providing an experimental evidence underpinning the relevant therapeutic implication.Methods Immunized guinea pigs were divided into two treatment groups versus two control groups by randomization. One of the treatment groups was given pentoxifylline(30mg/kg·d,im) and the other bromocriptine (5mg/kg·d, oral administration); the control groups were given the same volume of sterile saline or 5% glucose solution. Observation was made on four aspects:histological examination,activity of serum TNF,activity of serum IL-6, and serum prolactin levels.Statistical evaluation was performed using two-tailed student's t test and correlation analysis.Results Compared with the controls, the pentoxifylline and bromocriptine groups had lower activity of TNF and IL-6, lower level of serum prolactin, and lower incidence and severity of acute EAE.Conclusion The preventive effect of pentoxifylline on acute EAE might be mainly related to inhibition of the activity and secretion of TNF,and the effect of bromocriptine might be related to inhibition of prolactin secretion. These suggest further researches as well as clinical trials on the use of pentoxifylline in treating acute and relapsing multiple sclerosis.

关 键 词：实验性变应性脑脊髓炎 治疗 细胞因子 

分 类 号：R744.3[医药卫生—神经病学与精神病学]