肺癌患者外周血中表面活性蛋白D mRNA的表达及临床意义  

Expression and Clinical Significan ce of Surfactant Protein D mRNA in Peripheral Blood of Lung Cancer Patients

作　　者：齐之丽[1] 肖琅[2] 高英堂[1] 杜建民[2] 景丽[1] 

机构地区：[1]天津市第三中心医院分子细胞室,天津300170 [2]天津市第三中心医院呼吸内科,天津300170

出　　处：《癌症》2002年第7期772-775,共4页Chinese Journal of Cancer

基　　金：天津市卫生局科技基金项目(98KYGG-7)

摘　　要：背景与目的:循环癌细胞的灵敏检测对于肺癌患者的预后及选择合适的治疗方法很重要。为此,我们建立了利用表面活性蛋白D(surfactantproteinD,SP-D)作为基因标志的RT-PCR方法,检测肺癌患者外周血中的癌细胞,并初步探讨其临床意义。方法:采用优化的套式RT-PCR方法,对26例伴肺外转移肺癌患者,37例无转移肺癌患者,15例良性肺疾病患者及15例健康志愿者外周血中SP-DmRNA的表达进行分析。结果:1)该方法灵敏度可达1×10-6,特异性强;2)采用此方法,伴肺外转移和无转移肺癌患者外周血中SP-DmRNA的检出率分别为92.3%(24/26)和24.3%(9/37),所有良性肺疾病患者和健康对照外周血中均未见SP-DmRNA的表达。结论:SP-DmRNA可能是检测肺癌患者外周血中是否存在癌细胞的一个有价值的指标,对肺癌的转移倾向有一定的提示作用,并有可能为制定治疗方案和评估预后提供重要的参考依据。Background and Objectives:The sensitive detection of circulating tumor cells in lung cancer patien ts is very important for prognosis and selection of appropriate treatment mod alities.Based on recent promising d ata,the authors established reverse transcriptase(RT)-PCR with primers specific for surfa ctant protein D(SP-D)gene to detect circulating tumor cells in the peripheral blood o f lung cancer patients,and to initia tively discuss its clinical signifi cance.Methods:The expression of SP-D mRNA was analy zed by an improved nested RT-PCR in th e peripheral blood of 26lung cancer patients with metastases,37lung ca ncer patients without metastases,15benign pneumonia patients,and 15h ealthy volunteers.Results:1)The sensitivity of the method was 1×10 -6 ,with high level of specificity.2)Using this method,the positive detection rate of the ex pression of SP-D mRNA was 92.3%(24/26)and 24.3%(9/37)in the peripheral blood of lung cancer patients with and with out metastases,respectively.While no sample was positive for SP-D mRNA expression in the benign pneumonia patients and in the healthy volunteers.Conclusions:SP-D mRNA might be a valuable marker t o detect circulating tumor cells in th e peripheral blood of lung cancer patients.This method may be a valuable t ool for early identification of metastases in asy mptomatic lung cancer patients.Fur thermore,it can also provide important clinical information for the evaluation of pr ognosis and in the selection of appro priate treatment strategies.

关 键 词：表面活性蛋白D 肺肿瘤 循环癌细胞 RT-PCR SP-D MRNA 

分 类 号：R734.2[医药卫生—肿瘤]