鸭病毒性肝炎实验室诊断方法的研究  

The Method to Diagnose DHV

作　　者：秦智锋[1] 李环玲 贺东生[3] 刘福安[3] 

机构地区：[1]深圳出入境检验检疫局动植食中心动检室,广东深圳518000 [2]香港英特威有限公司广州办事处 [3]华南农业大学动物医学系,广东广州510642

出　　处：《中国预防兽医学报》2002年第4期310-313,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：将鸭病毒性肝炎病毒 (duckhepatitisvirusDHV) (ATCC株 )用鸡胚传一代后 ,测出此病毒的鸡胚半数致死量 (ELD50 )为10 -5.5。通过多次重复试验 ,建立了利用鸡胚中和试验和雏鸭中和试验检测鸭病毒性肝炎 (DVH)的诊断方法。 7株DHV毒株在鸡胚中和试验中的结果是 :胚传一代尿囊液死亡率为 2 / 8～ 5 / 8,1∶10血清中和保护率为 8/ 8,1∶10 0 0肝病料死亡率为 3/ 8～8/ 8,1∶10血清中和保护率为 8/ 8,正常对照全部存活。雏鸭中和试验的结果是 :2 4小时内 1∶10肝病料死亡率为 3/ 8～ 8/ 8,1∶5血清中和保护率为 8/ 8。A duck hepatiis virus (DHV)was propagated in chicken embryo,the median lethal dose(MLD)in chicken embryo of DHV was measured to be 10 -5.5 .The results of chicken embryo neutralization test of DHV were:The lethal rates of chorioallanoic fluid were among 2/8～8/8,the protection rates of ten fold diluted sera was 8/8;the lethal rates of infected liver supernatant which were diluted 1∶1 000 were among 3/8～8/8,the serum protection rates of ten fold diluion was 8/8; and the control were all normal.The results of duckling neutralization test were:the lethal rates of infected liver supernatant which were diluted 1∶1 000 were 3/8～8/8,the serum protection rates of five fold dilution was 8/8;and the control were all normal.So a set rules were developed to quarantine DHV by chicken embryos and young ducks,which will help to control and quarantine DHV.

关 键 词：鸭病毒性肝炎 鸡胚中和试验 雏鸭中和试验 

分 类 号：S852.53[农业科学—基础兽医学] S858.32[农业科学—兽医学]