釉基质蛋白对成骨细胞矿化及粘附能力的影响  

The Studies on Osteoblasts' Mineralization and Attachment Affected by Enamel Matrix Proteins

作　　者：邬春兰[1] 束蓉[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学院口腔内科,上海200011

出　　处：《上海口腔医学》2002年第2期135-137,共3页Shanghai Journal of Stomatology

基　　金：上海市卫生局科研基金(01411)

摘　　要：目的观察釉基质蛋白(EMPs)对成骨细胞矿化和粘附能力的影响,进一步探讨EMPs促进牙槽骨再生的机理。方法在培养液中加入不同浓度的EMPs,体外培养MC3T3-E1成骨细胞,用Von-Kossa染色法检测矿化结节的形成情况,粘附细胞计数法检测细胞的粘附能力。结果EMPs能明显促进MC3T3-E1成骨细胞的矿化能力(P<0.01),但对细胞的粘附能力无明显影响(P>0.05)。结论EMPs能提高成骨细胞的成骨能力,对成骨细胞在根面的定植无影响,提示这可能是其促进牙槽骨再生的一个重要原因。Objective Investigate the effects of enamel matrix proteins(EMPs) on the mineralization and attachment of osteoblasts,to explore the possible mechanism of alveolar bone regeneration induced by enamel matrix proteins(EMPs).Methods MC3T3-E1 osteoblasts were cultured in vitro and EMPs was added to medium in several concentrations. Cell's mineralizing ability was detected by Von-Kossa stain and attachment ability was detected by counting cells number. Results The results manifested that EMPs promote more mineral nodules formation (P<0.01). But MC3T3-E1 cells' attachment ability was not improved (P>0.05). Conclusions EMPs enhanced the mineralizing function of osteoblasts, had no effects on implanting on root surface of osteoblasts, which may play an important role in the regeneration of alveolar bone.

关 键 词：釉基质蛋白 成骨细胞 矿化 细胞粘附能力 牙槽骨再生 体外培养 

分 类 号：R780.2[医药卫生—口腔医学]