乳癌前哨淋巴结中mammaglobin检测的临床意义  

Clinical signipicance of mammaglobin detection in sentinel lymph nodes of breast cancer

作　　者：邬一军[1] 周新惠[1] 黄钟英[1] 于吉人[1] 郑树森[1] 

机构地区：[1]浙江大学医学院附属第一医院普外科,浙江杭州310003

出　　处：《中国普通外科杂志》2002年第6期328-330,共3页China Journal of General Surgery

摘　　要：目的　探讨乳癌前哨淋巴结中mammaglobin检测的临床意义。方法　 3 2例乳癌患者术中注射亚甲蓝定位前哨淋巴结 (SLN ) ,巢式RT -PCR法检测腋淋巴结中mammaglobinmRNA的表达。结果　SLN定位成功率为 93 .8% (3 0 /3 2 ) ,SLN与非SLN组微转移检出率有显著统计学差异 (P <0 .0 1) ;在常规病检阴性的淋巴结中 ,巢式RT PCR法的微转移的检出率为 14 .4% (3 8/2 63 )。结论　mamma globin巢式RT PCR法是较常规病理检查更为敏感的检出淋巴结转移的方法。联合SLN定位和巢式RT PCR的检测 ,可明显提高乳癌腋淋巴结微转移的检出效率。Objective To investigate the clinical significance of detecting mammaglobin in sentinel lymph nodes(SLN) of breast cancer. Methods In 32 breast cancer patients, methylene blue was injected around parechyma of breast cancer to locate the SLNs, and nested RT PCR(reverse transcriptase polgmecane chain reaction) was used to examine the expression of mammaglobin mRNA. Results The SLN was successfully identified in 30 of the 32 cases(93.8%).The micrometastases detection between group SLNs and non SLNs had significant statistical difference(P<0.01).Among the negative lymph nodes detected by routine pathologic examination, the detection rate of micrometastases was 14.4%(38/263) by nested RT PCR. Conclusions The mammaglobin nested RT PCR is more sensitive than routine pathologic method for detection of micrometastases. The combined utilization of locating SLN and nested RT PCR can greatly enhance the efficiency of micrometastases detection in axillary lymph nodes.

关 键 词：乳癌 前哨淋巴结 mammaglobin检测 临床意义 亚甲蓝 巢式RT-PCR法 

分 类 号：R737.9[医药卫生—肿瘤]