自身细胞因子诱导的杀伤细胞过继性免疫治疗恶性肿瘤的临床观察  

Clinical Observation on Adoptive Immunotherapy with Autologous Cytokine-Induced Killer Cells for Advanced Malignant Tumor

作　　者：陈复兴[1] 刘军权[1] 张南征[2] 巩新建[1] 张国龙[2] 徐永茂[2] 周忠海[1] 王涛[1] 黄健[3] 

机构地区：[1]中国人民解放军第97医院实验科,江苏徐州221004 [2]中国人民解放军第97医院消化肿瘤科,江苏徐州221004 [3]徐州医学院附属医院中心实验室,江苏徐州221002

出　　处：《癌症》2002年第7期797-801,共5页Chinese Journal of Cancer

摘　　要：背景与目的:细胞因子诱导的杀伤细胞(CIK细胞)具有增殖快、杀瘤活性强和杀瘤谱广的特点。但用于临床肿瘤治疗的报告较少,本文旨在评估用CIK细胞过继回输治疗晚期肿瘤的疗效。方法:用淋巴细胞分离液分离患者末梢血单个核细胞,然后将末梢血单个核细胞加入含有IFN-γ、IL-2和CD3单抗的培养基中进行培养扩增,再将培养后的CIK细胞回输给病人;患者一个疗程接受CIK细胞总数在5×109～15×109之间。在CIK细胞治疗前有47例患者做了化疗,3例做了放疗。化放疗与CIK细胞治疗间隔时间在2～4周以上。结果:在63例接受治疗者中,部分缓解(PR)+微效(MR)为28例(44.46%)。20例CEA增高者,治疗后有14例减低,6例增加;10例AFP增高者,治疗后有9例减低,1例增加。CD3、CD4和CD8T淋巴细胞绝对值在CIK细胞治疗后均增加45%以上。治疗后63例中有51例患者有食欲增加、32例患者睡眠和体力改善,18例患者中有13例疼痛减轻,结论:用自身CIK细胞过继性回输治疗能明显提高癌症患者细胞免疫功能,改善临床体征,且无毒副作用。Background &Objective:Cytokine-induced killer(CIK)cells have the characteristics of ra pid proliferation,high efficiency,an d broad spectrum in killing of tumor c ells.However,there was few report a bout its clinical application on treatment for cancer patients.The current study was designed to evaluate the effect of adopti ve transfer of autologous CIK cells on the patients with advanced malignant tumor.Methods:Peripheral blood mononuclear cells of the patients with advanced malignant tu mor were separated by fractionation on Ficoll-Hypaque gradient,then cu ltured in the medium containing IFN-γ,IL-2,and CD3McAb for 7days in vitro,and than the cultured auto-CIK cell s were transfused back to the patients.The numbers of t ransferred CIK cells per patient were 5-15×10 9 in one course of treatment.Among these patients,47cases received ch emotherapy,3cases received radiotherapy before CIK cells transfusion.The interval between chemoradiotherapy and immu notherapy was over 2to 4weeks.Results:Among 63patients receiving CIK cell s immunotherapy,the total effective rate(PR +MR)was 44.46%(28).In the patients with increasing of CEA level in serum,14cases showed reduction of serum CEA a nd 1cases remained increasing after the treatment with CIK cell.In the patients with increasing of AFP level in serum,sim ilarly,9cases showed reduction of s erum AFD and 1case remained increasing.The absolute members of CD3,CD4,and CD8T cells increased to over 45%after being treated with CIK cells.Among tre ated patients,the appetite of 51cases an d performance and sleep of 32cases got improved.Among 18cases,13cases s howed the pain relief.Conclusion:Adoptive immunotherapy of auto-CIK cells can significantly enhance cellular immune functions and improve subjective symptoms,but without side effects,so this is a sa fe and effective treatment for the patients with malignant tumor.

关 键 词：肿瘤 免疫治疗 过继性 细胞因子 杀伤细胞 

分 类 号：R730.5[医药卫生—肿瘤]