膀胱癌组织βhCG mRNA及其亚型表达分析  

Expression of human chorionic gonadotropin β genes in the tissues of bladder cancer

作　　者：姜永光[1] 曾甫清[1] 肖传国[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院泌尿外科,武汉430022

出　　处：《中华泌尿外科杂志》2002年第7期431-433,共3页Chinese Journal of Urology

摘　　要：目的　探讨 βhCG基因表达与膀胱肿瘤临床生物学的关系。 　方法　采用RT PCR法 ,结合限制性内切酶分析对 37例膀胱癌组织进行 βhCGmRNA及其亚型表达的检测。 　结果　 37例癌组织中 βhCGmRNA阳性 2 6例 (70 % )。阳性表达率与肿瘤病理分级无关 (P >0 .0 5 ) ;浸润性肿瘤 (13/ 14)较浅表性肿瘤 (13/ 2 3)阳性率高 (P <0 .0 1) ;浅表性肿瘤主要表达 β7基因 ,而浸润性肿瘤多同时表达 3个或以上 βhCGmRNA亚型。 　结论　膀胱肿瘤 βhCGmRNA表达与肿瘤临床分期相关。同时表达多个 βhCGmRNA亚型提示肿瘤分化差 ,易浸润。Objective To investigate the relationship between βhCG genes expression and the clinicobiological behavior of bladder cancer. Methods RT-PCR method combined with restriction endonuclease analysis was adopted to detect the expressions of βhCG mRNA and its subtypes in 37 cases of bladder cancer tissues. Results 26 of 37 cancer tissues (70%) were positive for βhCG mRNA expression. The positive rate was higher in invasive tumor (T 2～T 4,13/14) compared with that in superficial tumor (Ta～T 1,13/23) ( P <0.01), but not related to tumor grades ( P >0.05). Most of superficial tumors only expressed β7 gene, while invasive tumor expressed β7,β5, β3, or β8 . Conclusions The positive rate of βhCG mRNA expression is related to tumor stage. The expression of hCG gene β5, β3 or β8 in addition to β7 indicates a worse differentiated or advanced bladder tumor.

关 键 词：膀胱癌 MRNA 亚型表达 检测 膀胱肿瘤 绒毛膜性腺激素 

分 类 号：R737.14[医药卫生—肿瘤]