高强度超声治疗对肿瘤组织内酶活性的影响  

Effect of High Intensity Ultrasound Treatment on the Enzyme Activity in the Tumor

作　　者：常淑芳[1] 朱深银[2] 胡凯[3] 伍烽[3] 王智彪[3] 顾美礼[1] 

机构地区：[1]重庆医科大学第二临床学院妇产科,重庆市400010 [2]重庆医科大学附一院临床药理研究室 [3]重庆医科大学医学超声工程研究所

出　　处：《中国超声医学杂志》2002年第7期486-488,共3页Chinese Journal of Ultrasound in Medicine

基　　金：国家"九五"重点科技攻关项目 (96- 0 5- 0 2 - 0 1 );自然科学基金资助(39770 841 )内容

摘　　要：目的 :探讨高强度超声 (HIU)治疗对肿瘤组织内酶活性的影响。方法 :新西兰大白兔 5只 ,HIU(声强 5 0 W/cm2 ,频率 7.5 MHz) ,治疗后即刻切取肿瘤组织 ,分别采用 Pearson法 ,硫化铅法及钙钴法对辐照区及未辐照区进行染色 ,观察组织内 SDH,G- 6 - P及 AL P活性变化。结果 :HIU辐照区周围肿瘤组织 SDH、 G- 6 - P、 AL P为阳性 ,HIU辐照区组织 SDH、 G- 6 - P、 AL P均为阴性。结论 :HIU治疗后肿瘤组织内 SDH、 AL P、 G- 6 - P活性丧失 ,肿瘤细胞功能不可逆丧失 。Objective:To evaluate the enzyme activity of the tumor after high intensity ultrasound(HIU)treatment.Methods:Five New Zealand rabbits with endometrial VX 2 carcinoma 14 days after neoplasm implanted were used in the experiment.The rabbits underwent HIU at an acoustic intensity of 50 W/cm 2 with frenquency 7 5MHz.After treatment,the tumor were stained for the activity of succinodehydrogenase(SDH),glucose 6 phosphatase(G 6 P)and alkaline phosphatase(ALP)by Person,lead sulfide and calcium cobalt methods respectively.Results:It were negative for SDH,ALP,G 6 P staining in the HIU treated tumor,but the tissue around it were positive for SDH,ALP,G 6 P staining.Conclusions:After HIU treatment,the enzymes are inactivited and cell function is irreversibly lost in the HIU treated tumor,but the tissue around it are relatively safe.

关 键 词：高强度超声治疗 肿瘤 酶活性 治疗 

分 类 号：R730.5[医药卫生—肿瘤]