肾上腺素及地塞米松对白内障患者晶状体上皮细胞增殖的影响  

EFFECTS OF ADRENALINE AND DEXAMETHASONE ON PROLIFERATION OF THE LENS EPITHELIAL CELLS

作　　者：何涛[1] 惠延年[2] 王琳[2] 马吉献[2] 

机构地区：[1]解放军第322医院 [2]第四军医大学西京医院

出　　处：《解放军医学杂志》2002年第7期623-624,共2页Medical Journal of Chinese People's Liberation Army

摘　　要：为了观察肾上腺素及地塞米松对白内障患者晶状体上皮细胞 (lensepithelialcell,LEC)增殖的影响并探讨其机制 ,在行白内障超声乳化术中 ,取出中央 5～ 7mm的晶状体前囊 ,将其均分成 3等份 ,用于对照组及两实验组 ,分别培养于 10 -6mol/L的肾上腺素及地塞米松 4 8h,免疫组织化学染色后 ,采用医用多功能图像分析系统测定增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)阳性面积率。结果显示 ,10 -6mol/L的肾上腺素及地塞米松对LEC的增殖有明显的抑制作用 ,PCNA的阳性面积率分别为 (2 6 14± 0 92 2 ) %和(3 338± 0 838) % ,与对照组比较 ,差异有显著性意义 (P <0 0 1)。研究表明 ,肾上腺素及地塞米松对白内障患者LEC有明显的抑制作用 ,从而可用于白内障术后防治后囊膜混浊的发生 ,为临床筛选防治后发性白内障的药物提供了科学依据。To detect the effects of adrenaline and dexamethasone on proliferation of the lens epithelial cells and discuss the mechanism, the central area 5～7mm in diameter of the anterior capsule was obtained in cataract surgery (phacoemulsification, Phaco), and the specimens were divided into three parts, and were cultured in vitro and treated with 10 -6 mol/L adrenaline and dexamethasone for 48 hours. And then the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry was estimated by medical color image analysis. The data were analyzed with the statistical software. The results indicated that the 10 -6 mol/L adrenaline and dexamethasone had obvious inhibition on LEC proliferation. The positive area ratio of PCNA was (2 614±0 922)%( t =5 594, P =0 0000) and (3 338±0 838)%( t =3 361, P =0 0013). It is suggested that adrenaline and dexamethasone could inhibit the proliferation of LEC and could be used to treat PCO after cataract surgery. The experiments provide the scientific basis for selection of drugs to prevent PCO after cataract.

关 键 词：细胞增殖 白内障 晶状体上皮细胞 肾上腺素 地塞米松 增殖细胞核抗原 

分 类 号：R776.1[医药卫生—眼科] R977.1[医药卫生—临床医学]