基因座控制区元件HS2、HS3对β-珠蛋白基因表达的调控  

A Study of the Effect of Locus Control Region Elements HS2 and HS3 on β-globin Gene Expression

作　　者：贾春平[1] 黄淑帧[1] 曾溢滔[1] 

机构地区：[1]上海市儿童医院上海医学遗传研究所,上海200040

出　　处：《Acta Genetica Sinica》2002年第7期565-570,共6页

基　　金：国家自然科学基金 (No.3 9893 3 2 0 )资助项目~~

摘　　要：用增强型绿色荧光蛋白 (EGFP)为报道基因 ,通过构建不同的真核表达载体 ,并用脂质体转染法将其转染到K5 6 2及MEL细胞中 ,经流式细胞仪检测、半定量RT PCR及荧光倒置显微镜下观察荧光表达情况 ,以研究HS2、HS3、HS2 HS3及近侧元件在瞬时表达中对 β 珠蛋白基因启动子驱动下的EGFP表达调控情况。结果显示 ,3 2kb的HS2元件在K5 6 2及MEL细胞中均可增强 β 启动子活性 ,而 3kb的HS3仅在MEL细胞中有增强作用 。HS2 and HS3 are important elements of human β globin locus control region (LCR) . To study the effect of HS2 , HS3 , and HS2 HS3 on human β globin gene expression, a series of exprssion cassettes were constructed, in which the reporter gene encoding the enhanced green fluorescent protein ( EGFP ) was driven by the β globin promotor and under the control of HS2 , HS3 , and HS2 HS3 . These constructs were transfected transiently into MEL and K562 cell lines mediated with liposome and their expression was measured with FACS as well as semi quantitive RT PCR method. The results showed that 3.2 kb HS2 could significantly enhance the activity of β globin promotor in MEL and K562 cells, while 3 kb HS3 could do only in MEL cells. There was no synergistic function in transient expression in the cassettes with the combination of HS2 and HS3 in MEL and K562 cells.

关 键 词：基因座控制区元件 Β-珠蛋白基因 表达调控 

分 类 号：Q756[生物学—分子生物学]