应用PRINS技术定位黄鳝Hox基因的研究  

Chromosomal Localization of Rice Field Eel Hox Genes by PRINS

作　　者：戢福云[1] 刘江东[1] 易梅生[1] 黄琳[1] 周菲[1] 余其兴[1] 

机构地区：[1]武汉大学生命科学学院

出　　处：《Acta Genetica Sinica》2002年第7期612-615,共4页

基　　金：国家自然科学基金 (No .3 9970 40 6);高等学校博士学科点专项科研基金 (1999)项目资助

摘　　要：Hox基因是近年来发现的支持基因组复制进化理论的最有力的证据。该研究应用引物原位延伸 (PRINS)技术 ,在黄鳝有丝分裂染色体标本上开展Hox基因的定位研究。研究结果表明 ,在黄鳝染色体组中可能存在有 6个Hox基因簇 ,这些基因簇分别位于黄鳝第 1、2、3、6、8和 10号染色体 ,相对着丝粒距离分别为 2 8 2 4± 2 88、4 5 5±1 39、13 89± 2 0 3、74 32± 1.86、38 0 3± 2 .4 1和 5 8 18± 2 0 5。该研究定位黄鳝Hox基因将有助于挖掘黄鳝染色体的来源和进化特征 ,并为基因组复制进化理论提供黄鳝这一特化物种的细胞遗传学证据。The genome duplication and chromosome rearrangement are two kinds of evolution models at the chromosome level during the evolution of vertebrate genome. And Hox genes are the powerful proves to support the evolution theory of genome duplication, which has been found recently. In this study, the chromosomal localization of rice field eel Hox genes has been carried out by PRINS. The mapping results indicated that 6 Hox clusters might exist in rice field eel genome, and these clusters were localized on chromosome 1,2,3,6,8,10 and at the position of 28.24±2.88?4.55±1.39?13.89±2.03?74.32±1.86?38.03±2.41?58.18±2.05 from the centromere respectively. The mapping results that Hox genes were localized on chromosome 1,3,6 and 10 in the study are corresponding to that by chromosome microdissection. The chromosomal localization of rice field eel Hox genes will help us to discover the origin and evolution of rice field eel chromosomes, and provide cellular genetic proves of this special species to support the evolution theory of genome duplication.

关 键 词：黄鳝 HOX基因 PRINS 基因组复制进化理论 

分 类 号：Q349[生物学—遗传学]