受辐照窄颖赖草花粉DNA进入小麦胚囊的电镜自显影证据及杂种原位杂交鉴定  

EM AUTORADIOGRAPHY EVIDENCE FOR IRRADIATED POLLEN DNA OF Leymus angustus ENTERING WHEAT OVARY AND GISH OF ITS HYBRIDS

作　　者：李桂英[1] 王琳清[1] 施巾帼[1] 周荣华[2] 付仓生[1] 郝宏京[1] 

机构地区：[1]中国农业科学院原子能利用研究所 [2]中国农业科学院作物品种资源研究所,北京100081

出　　处：《核农学报》2002年第3期129-132,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金资助项目 (39770 4 97)

摘　　要：采用3H 胸腺嘧啶标记窄颖赖草 (Leymusangustus)花粉结合电镜自显影的方法证明受辐照窄颖赖草花粉DNA进入了小麦胚囊 ,运用基因组原位杂交技术证明所得杂种为真杂种 ,经辐照花粉获得的普通小麦J 1It was proved that DNA of irradiated pollen of Leymus angustus entered wheat ovary by means of labeling pollens of L.angustus with 3H\|TdR and electronic microscopic autoradiography.Genomic in situ hybridization analysis on wheat× L.angustus hybrids showed that chromosome number variation and structural aberration of L.angustus took place in the hybrids recovered after pollinating with irradiated pollens of L.angustus.

关 键 词：窄颖赖草 花粉DNA 小麦胚囊 电镜自显影 杂种 原位杂交鉴定 辐照花粉 基因组原位杂交 外源染色体 

分 类 号：Q945[生物学—植物学]