Effect of esculentoside A on autoimmunity in mice and its possible mechanisms  

商陆皂苷甲对自身免疫性模型小鼠的影响及其作用机制(英文)

作　　者：肖振宇 郑钦岳[1] 张俊平[1] 姜远英[1] 易杨华[2] 

机构地区：[1]第二军医大学药学院药理教研室,上海中国200433 [2]第二军医大学药学院天然药化教研室,上海中国200433

出　　处：《Acta Pharmacologica Sinica》2002年第7期638-644,共7页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China,№ 39470814.

摘　　要：AIM: To investigate the influence of esculentoside A (EsA) on autoimmunity in mice and its possible mechanisms. METHODS: The level of anti-ds DNA antibody, proliferation of lymphoid cells, and inflammation by pathologic section of joint in mice were examined. The autoimmunity model is made through immunizing mice with formaldehyde treated Campy-lobacter jejuni strain CJ-S131 and Freund's complete adjuvant. The apoptosis of T cell was analyzed through morphology and flow cytometry ( FACS ). The expression of ICAM-1 mRNA in human umbilical vein endothelial cell line (ECV304) was determined by coupled reverse transcription and PCR amplification (RT-PCR). RESULTS: EsA could potently lower the level of anti-ds DNA antibody, inhibit the proliferation of lymphoid cells, and ameliorate inflammation in the joint of model mouse. The apoptosis of thymocyte activated by ConA was markedly accelerated while the expression of ICAM-1 mRNA in ECV304 was decreased by EsA. CONCLUSION: EsA has the positive curative effect on autoimmunity in a mouse model, which may function through inhibition of expression of ICAM-1 mRNA in ECV304 and acceleration of thymocyte apoptosis.目的:考察商陆皂苷甲对自身免疫性模型小鼠的影响及其可能的机制.方法:自身免疫性小鼠模型通过甲醛化空弯菌CJ-S_(131)辅以佐剂免疫小鼠获得.检测正常小鼠、模型小鼠及商陆皂苷甲处理的模型小鼠血清中的抗ds DNA抗体水平、细胞增殖及骨关节病理切片.通过形态学和流式细胞仪检测胸腺细胞凋亡.通过逆转录PCR的方法检测ECV304细胞中ICAM-1的mRNA表达.结果:商陆皂苷甲能有效地降低模型小鼠的抗ds DNA抗体水平、抑制淋巴细胞增殖并能缓解其骨关节炎症.而且,商陆皂苷甲能显著促进ConA活化的胸腺细胞的凋亡并能降低ECV304 细胞中ICAM-1的mRNA表达.结论:商陆皂苷甲对自身免疫性模型小鼠有治疗作用;促进胸腺细胞凋亡和降低ECV304细胞中ICAM-1的mRNA表达可能是其作用的重要机制.

关 键 词：esculentoside A autoirnmunity INFLAMMATION intercellular adhesion molecule-1 APOPTOSIS rheumatoid arthritis 

分 类 号：R96[医药卫生—药理学]