大鼠卵巢经诊断超声照射后BCL-2表达的研究  

Research on the expression of BCL-2in rat ovary irradiated with ultrasonic wave

作　　者：曹敏丽[1] 王怀禄[1] 赵红斌[1] 尹满军 

机构地区：[1]兰州军区兰州总医院,甘肃兰州730050 [2]21军高炮旅卫生队,甘肃临夏731100

出　　处：《中国临床医学影像杂志》2002年第3期198-199,共2页Journal of China Clinic Medical Imaging

摘　　要：目的:分析大鼠卵巢经诊断超声照射后BCL-2表达的情况,旨在探讨大鼠卵巢细胞的凋亡情况。方法:将30只雌性大鼠随机分为6组:正常对照组、5min组、10min组、20min组、30min组和60min组。应用EUB-26黑白超声诊断仪予以不同时间持续照射,7天后断头即行取材。结果:超声持续照射大鼠卵巢10min内BCL-2呈低表达,20min时呈高表达,但随照射时间延长,BCL-2的表达随之减弱。结论:诊断超声持续照射大鼠卵巢20min后BCL-2表达增加,它在某种程度上可抑制由超声照射引起的细胞凋亡。Objective:To study the expression of BCL-2in rat ovary irradiated with ultrasonic wave and study apoptosis of ovarian cell.Methods:Thirty rats were randomly divided into six groups which contained the control group and5min,10min,20min,30min and60min irradiation groups.Rats'ovary were irradiated by ultrasonic wave.After seven days the rats were killed and the ovarian tissue was removed for detection.The immunohistochemistry technology was used to detect the expression of BCL-2in rat ovary.Results:The expression of BCL-2in the10min group was low,but it was higher in20min group than that of the other groups (P<0.05).The expression of BCL-2in30min and60min groups were not signifi-cantly different from that of the control and10min groups.Conclusions:BCL-2played an important role in inhibiting apop-tosis of ovarian cell caused by ultrasonic wave.

关 键 词：卵巢 淋巴瘤 B细胞 大鼠 经诊断超声照射 BCL-2 研究 

分 类 号：R737.31[医药卫生—肿瘤]