下咽癌细胞增殖细胞核抗原及其基因的表达研究  

The study of pression of proliferating cell nuclear antigen and its gene in hypopharyngeal carcinoma

作　　者：姚连生[1] 李湘平[1] 张进华[2] 

机构地区：[1]第一军医大学南方医院耳鼻咽喉科,广州510515 [2]第一军医大学南方医院病理科

出　　处：《临床耳鼻咽喉科杂志》2002年第7期346-348,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :通过对下咽癌细胞增殖细胞核抗原 (PCNA)及其基因的检测 ,以探讨其对下咽癌生物学特性、临床行为及预后的影响。方法 :用免疫组织化学染色SABC法检测 4 8例下咽癌手术标本和同期 1 5例癌旁正常组织标本PCNA的表达 ,计算其增殖指数 ;同时用寡核苷酸探针原位杂交技术检测PCNA的mRNA ,以检验免疫组化与原位杂交结果的一致性。结果 :下咽癌组PCNA增殖指数为 (71 .4 8± 1 1 .1 4 ) % ,对照组为 (2 1 .1 3±1 2 .0 7) % ,其差异有显著性意义 (P <0 .0 5 ) ;PCNA增殖指数与癌的临床分期、组织分化程度及预后相关 ,而与是否有颈淋巴结转移和肿瘤发生部位无明显关系 ,且免疫组化和原位杂交两种检测结果相一致 (符合率为 85 .7% )。结论Objective:To study the relationship of proliferating cell nuclear antigen (PCNA) and the biological and clinical characteristics of hypopharyngeal carcinoma and its value of clinical prognosis. Method:The SABC immunohistochemical method and in situ hybridization were correspondingly used to detect the expression of PCNA and its gene in 48 cases of cancer tissue and 15 cases of nearby cancer tissue of primary hypopharyngeal carcinoma.The number of positive cells of PCNA in 63 cases of tissue was represented by proliferating index (PI).Result:The expression of PCNA correlated with the clinical stage,histological grade and clinical recurrence of hypopharyngeal carcinoma and prognosis of the patients,but did not correlate significantly with tumor location and whether metastasis of cervical lymph nodes or not.Conclusion:The expression of PCNA protein may provide a reference in evaluating the malignancy level of hypopharyngeal carcinoma and prognosis of the patients.

关 键 词：下咽肿瘤 增殖细胞核抗原 免疫组织化学 原位杂交 

分 类 号：R739.63[医药卫生—肿瘤]