利用生物芯片技术探讨支原体与胃癌的关系  

Detection of Mycoplasma hyorhinis in gastric cancer using bio-chip technology

作　　者：张霁[1] 王怡[1] 寿成超[2] 徐光炜[1] 陈欣[3] 吴健[2] 谢玉泉[4] 李吉友[4] 苏启深 季加孚[1] 

机构地区：[1]北京大学临床肿瘤学院北京市肿瘤防治研究所外科,100036 [2]北京大学临床肿瘤学院北京市肿瘤防治研究所生化室,100036 [3]美国斯坦福大学 [4]北京大学临床肿瘤学院北京市肿瘤防治研究所病理科,100036

出　　处：《中华医学杂志》2002年第14期961-965,共5页National Medical Journal of China

基　　金：国家 973重点基础研究发展规划基金资助项目(G19980 5 12 0 3 ) ;北京市科委项目基金资助 (95 5 0 2 13 2 0 0 )

摘　　要：目的　探讨支原体感染与胃癌的相关性以及胃癌细胞感染支原体后基因表达的变化。方法　建立组织芯片制备体系 ,制备包含 10 5例胃癌组织、10 1例胃癌手术切缘正常组织和 6 2例胃良性疾患的组织芯片。以猪鼻支原体特异抗体PD4为一抗 ,利用免疫组织化学的方法对组织芯片进行染色 ,判断猪鼻支原体感染的分布情况。借助基因芯片分析人胃癌细胞系MGC80 3细胞被支原体感染后基因表达水平的变化。结果　被测标本中 ,5 4 1% (5 3 98)的胃癌组织存在猪鼻支原体感染。胃癌标本正常切缘组织感染率 5 1 7(45 87)。而胃良性疾患组织感染率仅为 15 8% (9 5 7) ,与胃癌组织相比差异有显著意义 (P =0 0 0 1)。感染率与肿瘤细胞分化程度成正比。人胃癌细胞MGC80 3细胞在感染支原体后 ,在被检测的 4 80 0 0个基因位点中共有 4 0 9个基因的表达水平发生明显改变。部分凋亡、细胞粘附相关基因的表达受到抑制。结论　胃癌组织中猪鼻支原体的感染率明显高于胃良性疾患组织 ,提示猪鼻支原体感染与胃癌的发生发展可能存在一定的相关性。猪鼻支原体的感染明显影响胃癌细胞的基因表达 ,提示猪鼻支原体的感染可能会影响胃癌细胞的生物学行为。Objective To determine the prevalence of Mycoplasma hyorhinis in archived paraffin embedded gastric cancer tissue and to find whether Mycoplasma hyorhinis infection can influence gene expression level in gastric cancer cells. Methods A high dense tissue microarray containing 105 gastric cancer samples, 101 benign margin samples and 62 non cancerous gastric disease samples resected during operation was constructed. PD4, a specific anti Mycoplasma hyorhinis Mab, was used to detect the infection rate in all the samples in the tissue microarrays immunohistochemically. Then, cDNA microarray was used to pinpoint defferentially the expressed genes between gastric cancer cell line MGC803 samples with and without Mycoplasma hyorhinis infection. Results The infection rate of M. hyorhinis was 54.1%(53/98) in gastric cancer samples, 51.7%(45/87) in benign margin samples, and 15.8 %(9/57) in non cancerous disease samples respectively. The difference of infection rates between gastric cancer and non cancerous gastric disease was statistically significant ( P = 0.001). Highly differentiated adenocarcinomas had more opportunity (84.6%) to be infected with M. hyorhinis than poorly differentiated ones (45.9%)( P <0.05). Intestinal type of gastric cancers (according to Lauren's classification) got the infection more often than diffused type. About 409 gene expression alterations were detected in 48 000 sites from two gastric cancer cell lines and the expression levels of some genes correlating with cell apoptosis and cell adhesion were down regulated after Mycopasma hyorhinis infection. Conclusion The infection rate of M. hyorhinis is significantly higher in gastric cancer than in other gastric diseases, thus indicating the association between Mycoplasma infection and gastric cancer. Mycoplasma hyorhinis infection influences the gene expression level in gastric cancer cell line MGC803, which indicates that the infection could have something to do with the process of gastri

关 键 词：生物芯片技术 支原体 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]