B23在肝再生和肝细胞增生过程中的表达与移位  

Expression and relocation of B23 in the process of rat liver cell hyperplasia and liver regeneration

作　　者：云径平[1] 刘宗石[2] 丘殷庆[2] 陈约翰[2] 

机构地区：[1]中山大学肿瘤医院,广州510060 [2]香港中文大学医学院

出　　处：《中华医学杂志》2002年第14期974-978,共5页National Medical Journal of China

摘　　要：目的　用B2 3蛋白的单克隆抗体研究B2 3表达在鼠肝大部分切除后再生过程中肝细胞的动态变化 ,探讨B2 3作为肝再生和肝细胞增生标记物的可能性。方法　鼠肝大部切除术后第 1、2、3、4和 7d收取再生肝组织 ,采用B2 3的单克隆抗体和Western印迹及免疫组化方法检测B2 3在不同时间再生肝组织的表达 ,并与增殖细胞核抗原 (PCNA)的表达相比较。结果　B2 3在静止期肝细胞中几乎检测不到 ,但在不同时间再生肝组织的表达中既有表达量的变化又有表达位置的变化 ,在术后 1～ 7d再生肝组织的表达中 ,B2 3表达量的增高变化呈近似抛物线样变化 ,其中第 3d表达水平最高 ,B2 3表达量的这些变化与PCNA的变化非常相似。B2 3表达位置的变化从核仁的增强表达 (G1 S)开始 ,进而核浆的表达 (S G2 )及胞质和分裂染色体的表达 (M)。结论　B2Objective To study the B23 expression and relocation in the process of liver regeneration at different time periods after partial hepatectomy (PH) on healthy rats, and to study the feasibility of using B23 as a proliferation marker. Methods Eighteen rats underwent partial hepatectomy (PH). The regenerative liver tissues were obtained at day 1, 2, 3, 4,and 7 after PH. The expression of B23 in hepatocytes of the regenerative tissues was measured using monoclonal antibody against nucleophosmin/B23 by Western blot and immunohistochemistry. The expression of proliferate cell nuclear antigen (PCNA) in these cells were measured using monoclonal antibody against PCNA by both methods for comparison with that of B23. Results Expression of B23 in the steady state of liver cells before PH was hardly detected by both methods. The increased expression of B23 in the regenerative liver cells was quantitatively changed like a parabolic curve at day 1 to days 7 after PH, with the peak expression of B23 at day 3 after PH. This quantitative variation of B23 expression was similar to that of PCNA expression. The variation of location of B23 in the regenerative liver cells was observed from nucleolus (G1 S phase) to nucleoplasm (S G2 phase) and to cytoplasm and mitotic chromosome (M phase). Conclusion B23 expression is quantitatively increased and moves in location in the process of liver regeneration after PH. B23 may be regarded as a proliferation marker in liver regeneration and liver cell hyperplasia.

关 键 词：核蛋白类 肝再生 细胞周期 免疫组织化学 

分 类 号：R575[医药卫生—消化系统]