微量热法研究硒代吗啉衍生物的抑菌作用  

Microcalorimetric Study on the Action of Selenomorpholine Derivatives on Growth Metabolism of Escherichia coli

作　　者：李曦[1] 刘义[2] 吴军[2] 屈松生[2] 

机构地区：[1]武汉理工大学材料复合新技术国家重点实验室,武汉430070 [2]武汉大学化学与分子科学学院

出　　处：《应用化学》2002年第7期645-648,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金 (2 9973 0 3 0 )资助项目

摘　　要：采用瑞典产 LKB-2 2 77生物活性检测系统 (热导式微量热计 )、用停流法研究了 3种硒代吗啉衍生物对大肠杆菌作用的热功率输出曲线 ,得到了大肠杆菌在不同药物浓度下的生长速率常数 k、半抑制浓度 IC50等参数 .结果表明 ,N -十二烷基硒代吗啉和 N -十二烷基硒代吗啉基乙酸甲酯溴化物的抗菌效果较明显 .The inhibitory or antibiotic actions of three kinds of the selenomorpholine derivatives on a strain of Escherichia coli have been studied by microcalorimetry. Differences in their abilities to inhibit the metabolism of this bacteria were observed. The extent and level of the inhibitory effect on the metabolism as judged by the rate constant, k , and the half inhibitory concentration, IC 50 , varied with the kinds of drugs. The rate constant( k ) of Escherichia coli in the presence of the drugs was found decreased with the increase in concentration of the drug. The experimental results revealed that the antibiotic activity of selenomorpholines was in order of: N dodecyl selenomorpholine acetyl methyl ester bromide> N dodecyl selenomorpholine> 4 selenomorpholine formyl ethyl ester.

关 键 词：硒代吗啉衍生物 大肠杆菌 生长代谢 抑菌作用 微量热法 药物化学 

分 类 号：R378.21[医药卫生—病原生物学] R914.4[医药卫生—基础医学]