幽门螺杆菌cagA菌株感染与胃癌组织中环氧化酶-Ⅱ表达的相关性研究  

Association between Helicobacter pylori cagA strain infection and expression of cyclooxygenase 2 in gastric carcinoma

作　　者：郭晓临[1] 王立娥[1] 袁媛[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所,沈阳110001

出　　处：《中华医学杂志》2002年第13期868-871,共4页National Medical Journal of China

基　　金：国家"九五"科研攻关课题资助项目 (96 90 6 0 1 0 4)

摘　　要：目的　探讨幽门螺杆菌cagA(Hp cagA)菌株感染与胃癌组织中环氧化酶 Ⅱ (COX 2 )表达之间的关系 ,为胃癌的早期预防提供有价值的实验和理论依据。方法　采用流式细胞术定量检测及分析 31例胃癌组织及相应的癌旁正常胃组织中COX 2的表达水平 ,并通过PCR技术对胃癌组织中cagA基因进行扩增检测。结果　 31例胃癌组织中 ,有 2 6例COX 2过表达 ,相应的 2 6例癌旁正常组织基本上无表达或弱表达。 18例cagA阳性的胃癌组织中COX 2的表达水平明显高于 13例cagA阴性的胃癌组织。结论　COX 2在胃癌组织中过度表达 ,cagA菌株感染可上调胃癌组织中COX 2的表达水平。可能存在cagA菌株感染之外的其他因素或机制调节COX 2的表达。因此 ,在根治Hp感染的同时 ,应用COX 2特异性抑制剂可能是一种有效预防胃癌发生。Objective To observe the expression of cyclooxygenase 2 (COX 2) in tissues of human gastric cancerr and explore the association between Helicobacter pylori cagA strain infection and the expression of COX 2 so as to provide a theoretical basis for early prevention of gastric cancer. Methods Flow cytometry was adopted to quantitatively determine and analyze the expression of COX 2 in 31 specimens of gastric cancer and normal juxta cancerous tissues. PCR was used to detect the cagA gene in gastric cancer. Results COX 2 was over expressed in 26 of the 31 (84%) specimens of gastric cancer. The expression of COX 2 in the 18 specimens of gastric cancer with cagA positive strain infection was significantly higher than that in the 13 specimens with cagA negative strain infection. Conclusion COX 2 is over expressed in gastric cancer and cagA positive strain infection up regulates the expression of COX 2 in gastric cancer. There may be another way or channel to regulate the expression of COX 2 in gastric cancer besides cagA positive strain infection. Therefore, applying COX 2 selective inhibitors to prevent gastric cancer can be an effective and promising way.

关 键 词：幽门螺杆菌 相关性 环氧化酶 胃肿瘤 细胞毒素类 基因表达 幽门螺杆菌感染 COX-2 

分 类 号：R735.2[医药卫生—肿瘤]