整合载体与选择载体共转化酵母菌的研究  

Multicopy integrating plasmid and selective plasmid co-transforming yeast

作　　者：侯爱华[1] 鲍晓明[1] 杨国梁[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《生物技术》2002年第3期5-6,共2页Biotechnology

摘　　要：以酿酒酵母菌株H158为例,研究了带有rDNA的多拷贝整合载体pMIRY2与选择载体pFAKnaMX4的共转化率,得到了共转化过程中两种载体的最佳比率为3-4:1,得到的共转化子在完全培养基中连续传代培养5d即可得到去除G418抗性标记基因的转化子,转化子的稳定性达到90%以上,实验表明该系统适合于外源基因在酵母中的稳定表达。Multicopy integrating plasmid pMIRY2 and yeast episomal plasmid pFAKnaMX4 which contains G418 resistance were co-transformed into yeast laboratory strain H158.The most efficient ratio was 3-4∶1.Transformants without G418 resistance can be obtained by continuously cultivating co-transformants in complete medium.The stability of the transformants was more than 90%.The experiment demonstrated that this co-transformation system is suitable for stabile expression of foreign genes in yeast.

关 键 词：整合载体 选择载体 共转化 酵母菌 

分 类 号：Q78[生物学—分子生物学]