小型猪肝硬化模型建立过程中肝脏功能及组织病理学的改变  

Functional and Histopathological Changes of Liver in Cirrhosis Model of Mini-pigs

作　　者：周忠信[1] 王捷[2] 罗葆明[2] 

机构地区：[1]广东省江门市中心医院外科,江门529000 [2]中山医科大学孙逸仙纪念医院普外科,广州510120

出　　处：《上海实验动物科学》2002年第2期102-105,97,共5页Shanghai Laboratory Animal Science

基　　金：广东省卫生厅科研基金 (E0 0 0 0 9710 2 );广州市重点科技攻关计划 (JB0 0 - 0 0 4 4 812 4 )

摘　　要：为观察小型猪肝硬化模型建立过程中肝功能及病理学改变 ,采用 CCl4 、苯巴比妥和无水酒精喂养小型猪共 1 2头 ,辅以高脂、低蛋白和低胆碱饮食 ,动态检测猪的肝功能并取组织行病理学检查。 4个月后 ,1 2头小猪成功诱导出肝硬化 ,病理学检查按 Madden分级 ,7头达到 级 ,5头达到 级。结果提示 ,CCl4 、苯巴比妥和无水酒精喂养辅以高脂低蛋白低胆碱饮食的多因素诱导小型猪肝硬化 ,成功率高 ,死亡率低 ,病理学改变分期界限明显。To determine the changes of function and histopathology of liver during developing cirrhosis in Mini-pigs, 12 Mini-pigs were fed with the food of high grease, low protein and choline, and carbon tetrachloride (CCL 4), fenobarbital and alcohol were added. Function and histopathology of liver were detected every 4 weeks. 12 pigs were all induced to form cirrhosis after 16 weeks, of which 7 pigs were grade Ⅲ and 5 grade Ⅳ, according to Madden standard. The results showed that the model established was easy to develop, with high rate of success, low rate of death, and obvious staging in histopathological changes.

关 键 词：小型猪 肝硬化 模型 肝脏功能 组织病理学 四氯化碳 

分 类 号：R-332[医药卫生]