尼莫地平对阿尔茨海默病大鼠脑组织NO、NOS含量及行为学的影响  

Effects of nimodipine on brain NO and NOS levels and behavioral science in Alzheimer disease rats

作　　者：白浩[1] 崔景彬[2] 王俊萍[2] 赵卫星[2] 祁颖[2] 

机构地区：[1]复旦大学医学院分子遗传学研究室,上海200032 [2]郑州大学基础医学院生物化学教研室,郑州450052

出　　处：《郑州大学学报（医学版）》2002年第4期428-430,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关资助项目 0 0 1170 80 5

摘　　要：目的 :探讨尼莫地平对阿尔茨海默病 (AD)大鼠脑组织一氧化氮 (NO)、一氧化氮合酶 (NOS)浓度及行为学改变的影响。方法 :SD大鼠 30只 ,随机分为空白对照组 ,AD模型组和尼莫地平组 ,采用大鼠脑组织立体定位微量注射技术 ,尼莫地平组和AD模型组用鹅膏蕈氨酸 (IBO) 1μl(5 μg)损毁大鼠双侧迈内特基底核 (nbM )建立AD动物模型 ,空白对照组以 0 .1mol/LpH7.4PB液代替IBO。尼莫地平组给予尼莫地平 0 .5mg/kg ,空白对照组和AD模型组以生理盐水代替尼莫地平 ,连续灌胃 6 0d ,每d 2次 ,每次 2ml。做迷宫试验及跳台试验测学习记忆能力 ,然后将大鼠断头处死 ,分离海马及大脑皮质 ,分别检测NO、NOS含量。结果 :AD模型组较空白对照组学习记忆能力显著降低 ,海马及大脑皮质NO、NOS含量显著升高 ;尼莫地平组较AD模型组学习记忆能力显著升高 ,海马及大脑皮质NO、NOS含量显著降低。结论 :用IBO损毁大鼠双侧nbM可建立AD动物模型 ,尼莫地平可显著改变AD模型大鼠的学习记忆能力 ,降低海马及大脑皮质NO、NOS含量。Aim: To determine the effects of nimodipine on brain nitric oxide (NO) and nitric oxide synthase (NOS) levels and behavioral science in Alzheimer disease (AD) rats. Methods: A total of 30 rats were allocated into control group, AD model group and nimodipine group (10 in each group). By the technique of solid-location and microinjection, in AD model group and nimodipine group, IBO 1 μl(5 μg)was injected into bilateral basal nuclei of Meynert (nbM)of rats to set up AD animal model. In control group IBO was replaced by 0.1 mol/L PB (pH 7.4). Nimodipine group rats orally took Nimodipine 0.5 mg/kg while control group and AD model group orally took normal saline. This process lasted for 60 days, twice per day, each time 2 ml.Then learning and remembering abilities were tested by maze experiment and jumping table experiment. Then all rats were decapitated, and hippocampus and cerebral cortex were dissected respectively to examine the concentrations of NO and NOS. Results: Compared with control group, in AD model group rats, the learning and remembering abilities decreased significantly, and the contents of NO and NOS increased significantly; compared with AD model group, in nimodipine group the learning and remembering abilities increased and the contents of NO and NOS decreased significantly. Conclusion: Ruining bilateral nbM of rats by IBO can set up ideal AD animal model. Nimodipine could significantly improve the learning and remembering abilities and reduce the contents of NO and NOS in hippocampus and cerebral cortex of AD model rats.

关 键 词：尼莫地平 阿尔茨海默病 大鼠 脑组织 NO NOS 含量 行为学 

分 类 号：R749.16[医药卫生—神经病学与精神病学]