高效液相色谱法测定肺癌患者尿中假尿嘧啶核苷和肌苷量比值  

Determination of urine pseudourine and trophicardyl in patients with lung cancer by high performance liquid chromatography

作　　者：吴逸明[1] 周舫[1] 吴拥军[1] 曹书霞[2] 刘一真[2] 

机构地区：[1]郑州大学公共卫生学院,郑州450052 [2]郑州大学化学系,郑州450052

出　　处：《郑州大学学报（医学版）》2002年第4期433-435,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关资助项目 991170 2 15

摘　　要：目的 :通过检测肺癌患者尿液中假尿嘧啶核苷和肌苷量比值 ,探讨其在肺癌诊断中的应用价值。方法 :肺癌患者、肺良性疾病及对照组各 5 0例 ,分别收集其晨起空腹尿液 ,离心过滤后利用高效液相色谱法同时检测假尿嘧啶核苷及肌苷 ,计算假尿嘧嘧与肌苷量比值。结果 :肺癌患者尿液中n (假尿嘧啶核苷 ) /n (肌苷 )比值有明显升高 ,与正常人比较差异有统计学意义 ,但肺良性疾病组与正常组和肺癌组比较 ,差异无统计学意义。结论 :假尿嘧啶核苷对肺癌诊断并非特异性肿瘤标志物 ,但可以作为肺癌早期诊断的一种辅助指标。Aim: To study the clinical value of the ratio of urine pseudourine vs trophicardyl in patients with lung cancer. Methods:A total of 50 cases of lung cancer,50 cases of pulmonary benign disease, and 50 controls were selected to determine the levels of pseudourine vs trophicardyl by high performance liquid chromatography.Results: The ratios of pseudourine vs trophicardyl were evidently higher in cases of lung cancer compared with controls, but there was no significant difference when comparing cases of pulmonary benign disease with cases of lung cancer or controls. Conlusions: Pseudourine is not specific tumor marker for lung cancer, but it can act as an assistant index for clinical diagnosis of lung cancer.

关 键 词：高效液相色谱法 测定 肺癌 尿 假尿嘧啶核苷 肌苷量 比值 

分 类 号：R734.2[医药卫生—肿瘤]