对氨基苯甲酸对牙龈卟啉单胞菌生长代谢影响的研究  

The effect of para-aminobenzoic acid on growth and metabolism of Porphyromonas gingivalis

作　　者：周学东[1] 王志凌 李继遥[1] 肖晓蓉[3] 胡涛[1] 

机构地区：[1]四川大学华西口腔医学院口腔内科,成都610041 [2]武警广东边防总队深圳医院口腔科 [3]四川大学口腔生物医学工程重点实验室

出　　处：《中华口腔医学杂志》2002年第4期275-277,I006,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目 (3 9670 785 )

摘　　要：目的　通过对氨基苯甲酸 (para aminobenzoicacid ,PABA)对牙龈卟啉单胞菌生长代谢的影响 ,研究血链球菌在龈下菌斑生态平衡中的作用。方法　在培养基中加入不同浓度的PABA ,对牙龈卟啉单胞菌行常规厌氧培养 ,测定培养后的细菌浓度吸光度 (A)值和胰酶样蛋白酶活性 ,并用扫描电镜观察菌细胞形态。结果　PABA能促进牙龈卟啉单胞菌的生长和胰酶样蛋白酶的活性 ,当PABA浓度为 1mg/L时 ,其促进作用最强 ,随着PABA浓度的升高 ,促进作用降低 ,当PABA浓度为 10 0mg/L时 ,无明显的促进作用。PABA还能对菌细胞的形态和粘附产生影响。结论　PABA能影响牙龈卟啉单胞菌的生长代谢 ,提示血链球菌可能对龈下菌斑的微生态平衡具有调节作用。Objective To study the effect of para aminobenzoic acid(PABA ) on the growth and metabolism of Porphyromonas gingivalis (P.g) . Methods After adding different concentrations of PABA into the medium, anaerobic technique was applied to culture P g . The products′ A value and action of TLP was assayed, and P g grew in the medium was observed by a scanning electron microscope. Results PABA promoted the growth of P g and action of TLP, which would reach the highest level when PABA was 1 mg/L, and would decrease with the increasing of concentration of PABA. When the concentration arrived at 100 mg/L, PABA had no effect on them. In the mean time, PABA had effect on the form and adherence of P g . When the concentration was 1 mg/L and 100 mg/L, this effect was strong, but as the concentration was 10 mg/L, the effect disappeared. Conclusions PABA influences the growth and metabolism of P g , which indicate that Streptococcus sanguis has regulative effect on the microecology of subgingival plaque.

关 键 词：对氨基苯甲酸 牙龈卟啉单胞菌 生长 代谢 研究 牙菌斑 

分 类 号：R780.2[医药卫生—口腔医学]