超临界CO_2流体萃取与分子蒸馏联用技术提取分离川芎挥发性成分及其GC/MS分析  

Analysis with gas chromatography and mass spectrography of volatile components of Ligus-ticum wallichii Franch extracted by CO_2 supercritical fluid extraction in combination with molecular distillation

作　　者：周本杰[1] 张忠义[1] 石勇[1] 古维新[1] 张守尧[1] 

机构地区：[1]第一军医大学珠江医院药剂科,广东广州510282

出　　处：《第一军医大学学报》2002年第7期652-653,共2页Journal of First Military Medical University

摘　　要：目的　研究超临界CO2流体萃取（SFE）与分子蒸馏（MD）联用技术对中药挥发性成分提取与分离、纯化的可行性。方法　采用超临界CO2流体萃取技术提取川芎的挥发性成分,再将所得到的挥发性成分进行分子蒸馏。以气相色谱（GC）-质谱（MS）技术分别测定萃取物和蒸馏物的化学成分,并对成分种类及其相对含量的变化进行比较。结果　川芎的超临界CO2流体萃取物共鉴定了45种成分,而超临界CO2流体萃取物再经分子蒸馏后所得蒸馏物的成分种类有所减少,为39种,两提取物所含成分的含量也有所变化。结论　SFE与MD联用技术可用于挥发性成分的分离、纯化,且优于单一SFE技术。Objective To evaluate the possibility of using the technique of CO2 supercritical fluid extraction (SFE) combined with molecular distillation (MD) to extract, separate and purify the volatile components of Ligusticum wallichii Franch, a Chinese herbal medicine. Methods SFE was employed to extract the volatile components of Ligusticum wallichii Franch, followed by MD of the product. Analysis of the chemical constituents of the extract both before and after MD was performed with gas chromatography (GC) in conjunction with mass spectrography (MS). Results There were 45 kinds of chemical constituents in the extract of Ligusticum wallichii Franch yielded from SFE, among which 39 were left after distillation by MD. Changes also took place in the content of the constituents in the extract after distillation. Conclusions SFE combined with MD, a technique better than simple SFE, can be used to extract, separate and purify the volatile components of Chinese herbs.

关 键 词：超临界CO2流体萃取 分子蒸馏 川芎 挥发性成分 GC/MS分析 

分 类 号：R284.2[医药卫生—中药学]