环氧合酶-2在子宫内膜癌组织中的表达  

Cyclooxyenase-2 expression in endometrium carcinoma

作　　者：李静[1] 卢运萍[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《中华妇产科杂志》2002年第7期408-410,W001,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然基金资助项目 (3 9840 0 0 9);国家杰出青年基金资助项目 (3 0 0 2 5 0 17)

摘　　要：目的　探讨环氧合酶COX 2与子宫内膜癌发生、发展的关系。方法　应用免疫组织化学、蛋白印迹法和逆转录聚合酶链反应 (RT PCR)技术 ,检测增生期内膜 2 5例 (增生期组 ) ,分泌期内膜2 5例 (分泌期组 ) ,内膜炎内膜 2 5例 (内膜炎组 ) ,非典型增生内膜 2 3例 (非典型增生组 ) ,子宫内膜癌34例 (内膜癌组 )组织中COX 2蛋白和mRNA的表达。结果　COX 2在增生期组、分泌期组、内膜炎组、非典型增生组、子宫内膜癌组的表达率分别为 :6 7、6 4、6 0、6 0、6 7% ,免疫组织化学染色强度分别为(5 4 6± 0 12 )、(3 2 0± 0 18)、(4 78± 0 12 )、(6 10± 0 2 5 )、(8 70± 0 93)分 ;COX 2蛋白表达水平分别为 0 75± 0 2 3、0 4 1± 0 4 5、0 5 6± 0 31、1 10± 0 5 6、1 4 6± 0 4 1;COX 2mRNA含量分别为 (93± 8)、(6 5± 11)、(79± 6 )、(2 99± 11)、(493± 30 )fpg μg。内膜癌组COX 2蛋白和mRNA表达强度明显高于其他 4组 ,差异均有极显著性 (P <0 0 0 1)。内膜癌组中高分化细胞COX 2表达高于低分化细胞 ,差异有显著性 (P <0 0 5 )。非典型增生组显著高于增生期组、分泌期组和内膜炎组 ,增生期组显著高于分泌期组 ,差异均有显著性 (P <0 0 5 )。结论　COX 2在子宫内膜癌的发生。Objective To investigate the expression of cyclooxyenase 2 (COX 2)in carcinogensis and development of endometrium carcinoma Methods Immunostainings, westernblotting and quantitve reverse transcription polymerase chain reaction (RT PCR) assay were utilized to measure levels of protein and mRNA expression of COX 2 in following five study groups: 25 cases with proliferative phase, 25 cases with secretory phase,25 cases with endometritis, 23 cases with atypical proliferative phase and 34 cases with endometrium carcinoma Results COX 2 expression of both RNA and protein in patients with endometrial carcinoma was higher significantly than patients with proliferative phase, secretary phase,endometritis,atypical proliferate phase Immunostaining score was 5 46±0 12 vs 3 20±0 18,4 78±0 12,6 10±0 25,8 70±0 93, average absorbent value was 0 75±0 23 vs 0 41±0 45, 0 56±0 31, 1 10±0 56,1 46±0 41;concentration of mRNA [(93±8) vs (65±11),(79±6),(299±11),(493±30) fpg/μg respectively] Successively the expression of COX 2 in atypical proliferation group was higher than normal endometrial and endometritis group The expression in proliferative phase group was higher significantly than secretory phase group ( P <0 05) Conclusion COX 2 may play an important role in the development of endometrial carcinoma

关 键 词：环氧合酶-2 子宫内膜癌 MRNA 免疫组织化学 临床意义 RT-PCR 蛋白印迹法 

分 类 号：R737.33[医药卫生—肿瘤]