Sorcin基因和mdr1基因的相关性及临床意义  

Expression and clinical implication of soluble resistance-associated calcium-b inding protein gene and multi-drug resistance gene in leukemia

作　　者：李光耀[1] 谭耀红[1] 杨纯正[1] 赵春华[1] 赵洪国[2] 王建祥[1] 薛艳萍[1] 韩明哲[1] 钱林生[1] 赵春亭[2] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所实验血液学国家重点实验室 [2]青岛大学医学院附属医院血液科

出　　处：《中华肿瘤杂志》2002年第4期370-374,共5页Chinese Journal of Oncology

基　　金：国家"九五"医学科技攻关基金资助项目 (96 90 6 0 1 2 3 )

摘　　要：目的　探讨急性髓细胞系白血病 (AML)患者可溶性耐药相关钙结合蛋白基因 (sorcin)和多药耐药基因 (mdr1)的相关性表达及其与临床耐药和疗效的关系。方法　Northernblot检测K5 6 2 /A0 2细胞株sorcin和mdr1基因的表达 ,用半定量逆转录聚合酶链反应 (RT PCR)检测 6 5例AML患者、2 7例非白血病患者和健康人的sorcin基因和mdr1基因表达 ,并分析sorcin基因和mdr1基因表达的相关性及其临床意义。结果　AML患者中 ,sorcin和mdr1基因表达明显高于正常对照组 ,差异有非常显著性 (P <0 .0 0 1) ;在AML和对照组中 ,sorcin和mdr1基因的表达有较好的相关性 ;sorcin和mdr1基因的共表达与AML患者的临床耐药和预后具有较高的符合率。Sorcin+ 和mdr1+ 组患者临床耐药发生率为 92 .9% ,完全缓解 (CR)率为 7.1% ;sorcin-和mdr1-组患者临床耐药发生率为 8.6 % ,CR率为91.4 %。结论　白血病患者的sorcin和mdr1基因共表达与其临床耐药和预后密切相关 ,可作为检测临床耐药和判断预后的有效手段之一。Objective To study the relationship between soluble resistance related calcium binding p rotein(sorcin) gene and multidrug resistance gene(mdr1), and their signific ance in clinical drug resistance and prognosis of acute myeloid leukemia(AML). Methods Amplification of sorcin gene and mdr1 gene in K562/A02 cell detected by Northern blot, were monitored by semi quantitative reverse transcription polymerase cha in reaction(RT PCR) in 65 AML patients and 27 normal controls, with their r elationship and clinical outcame analyzed. Results The amplification of sorcin gene and mdr1 gene in AML patients were significan tly higher than that in the normal control, which were related to clinical drug resistance and prognosis. The amplification of sorcin gene was related to th e amplification of mdr1 gene in the two groups. The clinical drug resistance in c idence rate and complete remission rate were 92.9% and 7.1% in sorcin +/mdr1 + group. They were 8.6% and 91.4% in the sorcin -/mdr1 - group( P <0.001 ). Conclusion The co amplification of sorcin and mdr1 gene can be taken as a good indicator of clinical drug resistance and prognosis of AML.

关 键 词：Sorcin基因 MDR1基因 临床意义 急性髓细胞系白血病 多药耐药 

分 类 号：R730.4[医药卫生—肿瘤]