Baclofen对大鼠新鲜分离DRG神经元GABA和NMDA激活电流的抑制作用  

Inhibitory Effect of Baclofen on GABA-and NMDA-Activated Currents in Neurons Freshly Dissociated from Rat Dorsal Root Ganglion

作　　者：赵义梅[1] 木拉提[2] 王英姿[1] 呼海燕[1] 司军强[1] 

机构地区：[1]石河子大学医学院医学系,新疆石河子832002 [2]石河子大学医学院第一附属医院,新疆石河子832002

出　　处：《石河子大学学报（自然科学版）》2002年第2期91-94,共4页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金资助项目 (3986 0 0 2 7)

摘　　要：用全细胞膜片箝技术在大鼠新鲜分离的背根神经节 (DRG)细胞上观察到baclofen对GABA和NMDA激活电流有调制作用。单独给予DRG细胞baclofen (1～ 10 0 μmol/L)未记录到可测的电流改变 ,但预加baclofen对GABA和NMDA激活电流具有明显的抑制作用 ,且呈剂量依赖性。 10 0 μmol/Lbaclofen对GABA和NMDA激活电流分别抑制(5 1.2± 10 .9) % (X±SE ,n=8,P <0 .0 1)和 (6 4 .1± 2 1.1) % (X±SE ,n=6 ,P <0 .0 1) ,此抑制作用是可逆的 ,可被GABAB 受体拮抗剂saclofen(10 0 μmol/L ) 所取消 (n =4 )。The whole cell patch clamp technique was used to record NMDA and GABA activated currents in neurons freshly dissociated from rat dorsal root ganglion neurons. It was shown that pre application of baclofen (1～100 μmol/L )could induce a concentration dependent inhibition of GABA and NMDA activated currents. GABA and NMDA activated currents were inhibited by (51.2±10.9)%(X±SE,n=8,P<0.01)and (64.1±21.1)%(X±SE,n=6,P<0.01)by 100 μmol/L baclofen , respectively. The inhibitory effect of baclofen was reversible, and could be abolished by GABA\-B receptor antagonist saclofen (100 μmol/L ).

关 键 词：BACLOFEN 大鼠 DRG神经元 激活电流 抑制作用 背根神经节 GABA受体 NMDA受体 调制作用 膜电流 

分 类 号：Q424[生物学—神经生物学]