动物细胞培养过程中pH和溶氧的关联控制  

Interactive Control of pH and DO in the Animal Cell Culture

作　　者：顾小华[1] 陈因良[1] 

机构地区：[1]华东化工学院生化工程研究所

出　　处：《华东化工学院学报》1989年第4期498-503,共6页

摘　　要：本文通过对动物细胞培养过程中pH值和溶氧(DO)水平控制要求的分析,设计了用空气、氧气、氮气和二氮化碳四组气体关联控制pH值和DO水平的控制系统。该系统根据对瞬时pH值和DO值参数的检测,通过微处理机进行一系列的逻辑判断和具有PI调节规律的DDC增量词节计算,再经四气体关联互补计算和多重时序控制环节,综合调节四组气体电磁阀的相互开闭时间,实现对培养过程中pH值和DO水平的控制,满足动物细胞生长的需要。A new design of interactive control scheme is presented for the regulation of pH and DO in the mass culture of animal cells. It is simply composed of four-gas (air, oxygen, carbon dioxide and nitrogen) coupling compensating calculation (CCC) and multiple-sequence-control (MSC). By a series of logic judgements of signals from sensors and direct digital calculations (DDC), a simple proportional plus integral (PI) controller can manipulate the operations of electrocegnetic valves to meet the requirements of pH and DO levels in the growth and metabolism of animal cells.

关 键 词：细胞培养 动物细胞 PH 溶氧 

分 类 号：Q954.6－332[生物学—动物学]