EB病毒对人胃癌细胞系HSC-39感染的研究  

Epstein-Barr virus infection of a human signet ring cell gastric carcinoma cell line

作　　者：罗兵[1] 村上雅尚[2] 柳原五吉 西连寺刚[2] 

机构地区：[1]青岛大学医学院微生物学教研室 [2]日本鸟取大学医学部生体情报学教室 [3]日本国立癌症研究中心实验动物管理室

出　　处：《中华微生物学和免疫学杂志》2002年第4期379-384,共6页Chinese Journal of Microbiology and Immunology

摘　　要：目的　探讨EB病毒 (EBV)对胃癌细胞系的感染作用。方法　用Akata和P3HR 1EBV毒株感染人胃癌印戒细胞系 (HSC 39) ,有限稀释法对感染细胞进行克隆。结果　EBV感染细胞中可检测到EBV编码的核抗原 (EBNA) ,2种EBV毒株感染的细胞克隆表现有不同的形态学特征及生长方式。EBV感染的亲代细胞及大部分克隆表达EBNA1,但不表达EBNA2、潜伏期膜蛋白 (LMP) 1和LMP2A ;亲代细胞及所有细胞克隆未观察到裂解感染 ,EBNA启动子Qp表达阳性 ,而启动子Cp和Wp未见表达。结论　HSC 39对 2种EBV毒株均易感 ,EBV感染可改变HSC 39的细胞表型 ,且不同EBV毒株对其影响不同 ,提示印戒细胞癌细胞系可用作EBV感染的靶细胞。Objective To study Epstein-Barr virus (EBV) infection on gastric carcinoma cells. Method A signet ring cell line HSC-39 derived from human gastric carcinoma was infected with Akata and P3HR-1 EBV strains. Akata and P3HR-1 EBV infected cell clones were isolated by a limiting dilution method. Results EBV-encoded nuclear antigen (EBNA) was detected in the infected cells. The Akata and P3HR-1 infected clones showed heterogenous morphology and growth pattern. The EBV infected parental cells and most clones expressed EBNA1, but not EBNA2, latent membrane protein (LMP) 1 and LMP2A. The Q promoter (p) but not Cp/Wp was active in the infected parental cells and all clones. No lytic infection was observed in the infected parental cells and all clones. Conclusion HSC-39 cells were susceptible to the both EBV strains. The phenotypes of HSC-39 cells were altered by EBV infection and the alteration depends on the EBV strains. This study defined a signet ring cell line as a new target for EBV with a unique infection.

关 键 词：EB病毒 胃癌 印戒细胞系 有限稀释法 

分 类 号：R735.2[医药卫生—肿瘤]